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Series GSE115937 Query DataSets for GSE115937
Status Public on Feb 13, 2019
Title Simultaneous quantification of antibody-RNA conjugates and the transcriptome from fixed cells by RAID
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Undifferentiated and differentiated Keratinocytes (AG1478 treated) were stained with antibody-RNA conjugates to measure protein-based diffrentiation changes in conjunction with single-cell transcriptomics. The cells were crosslinked and stained according to the RAID procedure to allow intracellular immunostaining. Antibodies used in this experiment are (TGM1, NOTCH1, KLK6, JAG1, phospho-RPS6, phospho-FAK).
 
Overall design Three 384 wells plates for untreated and Three 384 wells plates for AG1478 treated cells were processed for single cell transcriptomics
 
Contributor(s) Gerlach JP, Mulder KW
Citation(s) 30728416
Submission date Jun 18, 2018
Last update date Mar 26, 2019
Contact name Jan Patrick Gerlach
E-mail(s) janpgerlach@gmail.com
Organization name Radboud Institute for Molecular Life Sciences
Department Molecular Developmental Biology
Street address Geert Grooteplein Zuid 28
City Nijmegen
ZIP/Postal code 6500HB
Country Netherlands
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM3195605 Untreated_384wPlateA
GSM3195606 Untreated_384wPlateB
GSM3195607 Untreated_384wPlateC
This SubSeries is part of SuperSeries:
GSE115981 RAID allows multiplexed quantification of intracellular (phospho-)proteins and transcriptomes from fixed single cells
Relations
BioProject PRJNA476554
SRA SRP150759

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE115937_RAID_ARC_Rawcounts.csv.gz 26.8 Kb (ftp)(http) CSV
GSE115937_Transcripts_RAWcounts.tab.gz 6.9 Mb (ftp)(http) TAB
GSE115937_antibody_counts_SUB400.csv.gz 12.9 Kb (ftp)(http) CSV
GSE115937_mRNA_counts_SUB4500.csv.gz 3.0 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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