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Status |
Public on Jun 19, 2018 |
Title |
p53 binding and chromatin dynamics after etoposide treatment of primary lung fibroblast |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We report the chromatin modification dynamics at p53 binding sites afte 6 hours of treatment of etoposide (100 micromolar final) in IMR90 human lung fibroblasts using ChIP-seq , ATAC-seq, and polyA+ RNA-seq analyses. We assessed the genomewide occupancy of histone H3, H3K4me3, H3K4me2, H3K4me1, H3K27ac, H4K16ac, RNA polymerase II, p53, and transposase-accessible chromatin (ATAC-seq) in response to etoposide treatment.
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Overall design |
ChIP-seq, RNA-seq, and ATAC-seq for histone modifications, p53, and RNA polymerase II after etoposide treatment in IMR90 fetal lung fibroblasts
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Contributor(s) |
Sammons M |
Citation(s) |
30966857 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R15 GM128049 |
Molecular mechanisms regulating the establishment of cis-regulatory elements by the transcription factor p63 |
UNIVERSITY AT ALBANY, SUNY |
Morgan Andrew Sammons |
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Submission date |
Jun 18, 2018 |
Last update date |
Sep 13, 2019 |
Contact name |
Morgan A Sammons |
E-mail(s) |
masammons@albany.edu
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Organization name |
University at Albany - SUNY
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Department |
Biological Sciences
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Lab |
Sammons
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Street address |
1400 Washington Ave, Life Sciences 2075
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City |
Albany |
State/province |
NY |
ZIP/Postal code |
12222 |
Country |
USA |
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Platforms (2) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (11)
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Relations |
BioProject |
PRJNA476558 |
SRA |
SRP150762 |