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Series GSE115992 Query DataSets for GSE115992
Status Public on Feb 08, 2019
Title Small RNA sequencing of Barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei (Bgh); isolate 5874)
Organisms Hordeum vulgare; Blumeria hordei
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Purpose: The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012. Molecular Plant Pathology 13 (4): 414-430. DOI: 10.1111/j.1364-3703.2011.00783.x). Blumeria graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to identify small RNAs (sRNAs) from both barley and Bgh that regulate gene expression both within species and cross-kingdom.
 
Overall design 90 samples analyzed = 5 genotypes * 6 time points * 3 replications
Note: This experiment used the identical split-plot design, tissue, and source RNA as GEO submission # 101304 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE101304).
 
Contributor(s) Hunt M, Fuerst G, Wise RP
Citation(s) 31345162
Submission date Jun 19, 2018
Last update date Aug 06, 2019
Contact name Gregory Fuerst
E-mail(s) gregory.fuerst@usda.gov
Organization name USDA/ARS
Department CICGRU
Street address Department of Plant Pathology and Microbiology, Iowa State University, 1344 Advanced Teaching & Research Bldg., 2213 Pammel Dr.
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL23699 Illumina HiSeq 2500 (Blumeria graminis f. sp. hordei; Hordeum vulgare)
Samples (90)
GSM3204569 WT_t0_rep1
GSM3204570 WT_t0_rep2
GSM3204571 WT_t0_rep3
Relations
BioProject PRJNA476756
SRA SRP150840

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE115992_Exp20452_Barley_read_counts.csv.gz 76.0 Kb (ftp)(http) CSV
GSE115992_Exp20452_Bgh_read_counts.csv.gz 121.7 Kb (ftp)(http) CSV
GSE115992_Exp20452_Experiment_Design.pdf 42.3 Kb (ftp)(http) PDF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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