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Series GSE11616 Query DataSets for GSE11616
Status Public on Jul 01, 2008
Title rge1-1, retarded growth of embryo mutant vs wild-type Col-0
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The loss-of-function mutation of the RGE1 (retarded growth of embryo) gene, which is expressed during seed development, caused small and shriveled seeds. The embryo of the loss-of-function mutant showed retarded growth after the heart stage although abnormal morphogenesis and pattern formation of the embryo and endosperm was not observed. RGE1 expression was determined in endosperm cells using the β-glucuronidase reporter gene and RT-PCR. The differences of expression profile between rge1-1 and wild type Col-0 was examined to know the functions of RGE1. The result of Microarray analysis showed specific down-regulation of putative GDSL motif lipase genes in the rge1-1 mutant indicating possible involvement of these genes in seed morphology.
 
Overall design Total RNA was isolated from siliques of rge1-1 and wild type at 12 DAF using an RNAqueous Kit (Ambion, Inc, USA).
 
Contributor(s) Kondou Y, Nakazawa M, Kawashima M, Muto S, Matsui M
Citation(s) 18567831
Submission date May 30, 2008
Last update date Dec 06, 2012
Contact name Minami Matsui
E-mail(s) minami@riken.jp
Phone +81-45-503-9623
Fax +81-45-503-9586
Organization name RIKEN
Department Plant Science Center
Lab Plant Functional Genomics Research Team
Street address Suyehirocho 1-7-22 , Tsurumiku
City Yokohama
ZIP/Postal code 230-0045
Country Japan
 
Platforms (1)
GPL888 Agilent-011839 Arabidopsis 2 Oligo Microarray G4136A (Feature Number version)
Samples (2)
GSM294800 rge1-1 vs Col-0, rep1
GSM294801 rge1-1 vs Col-0, rep2
Relations
BioProject PRJNA106241

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11616_RAW.tar 2.5 Mb (http)(custom) TAR
Processed data included within Sample table

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