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Series GSE116299 Query DataSets for GSE116299
Status Public on Aug 22, 2018
Title A folic acid-enriched diet attenuates prostate involution in response to androgen deprivation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Folic acid is present in pre-natal vitamins, fortified cereal grains and multi-vitamin supplements. High intake of folic acid through these sources has resulted in populations with increased levels of serum folate and unmetabolized folic acid. Although the benefits of folic acid in the prevention of neural tube defects are undeniable, the impact of long-term consumption of folic acid on the prostate is not fully understood. In this study, we used a rodent model to test whether dietary folic acid (FA) supplementation changes prostate homeostasis and response to androgen deprivation. Although intact prostate weights do not differ between diet groups, we made the surprising observation that dietary folic acid supplementation confers partial resistance to castration-mediated prostate involution. More specifically, male mice that were fed a folic acid supplemented diet and then castrated had greater prostate wet weights, greater prostatic luminal epithelial cell heights, and more abundant RNAs encoding prostate secretory proteins compared to mice that were fed a control diet and castrated. We used RNA-seq to identify signaling pathways enriched in the castrated prostates from folic acid supplemented diet fed mice compared to control mice. We observed differential expression of genes involved in several metabolic pathways in the FA supplemented mice. Together, our results show that dietary FA supplementation can impact metabolism in the prostate and attenuate the prostate’s response to androgen deprivation. This has important implications for androgen deprivation therapies used in the treatment of prostate disease, as consumption of high levels of folic acid could reduce the efficacy of these treatments.
Overall design Ventral prostate lobes were collected from intact or castrated mice on one of two diet groups: control diet and folic acid enriched diet. A total of 23 samples from 6 treatment groups were profiled by RNA-Seq.
Contributor(s) Joseph DB, Vezina CM, Chu L
Citation(s) 30298636
NIH grant(s)
Grant ID Grant title Affiliation Name
R03 DK096074 The Epigenome: A New Target for Androgen Receptor in Developing Prostate UNIVERSITY OF WISCONSIN MADISON CHAD M. VEZINA
U54 DK104310 Role of Beta-Catenin in Urinary Dysfunction UNIVERSITY OF WISCONSIN MADISON CHAD M. VEZINA
Submission date Jun 26, 2018
Last update date Feb 11, 2019
Contact name Chad Michael Vezina
Organization name University of Wisconsin-Madison
Department Comparative Biosciences
Street address 1656 Linden Drive
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (23)
GSM3227732 CNTRL_Intact_rep1
GSM3227733 CNTRL_Intact_rep2
GSM3227734 CNTRL_Intact_rep3
BioProject PRJNA478050
SRA SRP151489

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Supplementary file Size Download File type/resource
GSE116299_mouseUGS_folic_acid_diet_EC.xls.gz 1.3 Mb (ftp)(http) XLS
Raw data are available in SRA
Processed data are available on Series record

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