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Series GSE116475 Query DataSets for GSE116475
Status Public on Feb 12, 2019
Title Gene expression analysis of WT and Stat1-/- CD4+ T cells pre and post transfer into Rag1-/- mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To understand the basis behind the defective survival/expansion of Stat1-/- CD4+ T cells in Rag1-/- mice, we analyzed the gene expression of WT and Stat1-/- CD4+ T cells pre- and post-transfer into these mice by RNAseq.
Overall design WT (C57BL/6) or Stat1-/- CD4+ T cells (3 donor mice/group) were MACS sorted by negative selection. Some of the T cells from each mouse was used for RNAseq (Pre-transfer, WT=1, KO=2). The remaining T cells were injected into Rag1-/- mice (5 mice/group) and FACS sorted from the spleens and lymph nodes (CD45+ CD3ε+ CD4+) 7 days later. In the post-transfer setting, the T cells from the 5 mice of each group were split into 3 biological replicates each containing 1-2 mice (denoted as WT or KO).
Contributor(s) Kang Y, Biswas A, Field M, Snapper SB
Citation(s) 30796216
Submission date Jul 01, 2018
Last update date Mar 25, 2019
Contact name Scott Snapper
Organization name Boston Children's Hospital
Department GI/Nutrition
Street address 300 Longwood Avenue, Enders 670
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM3239806 WT T Pre-transfer rep 1
GSM3239807 WT T Pre-transfer rep 2
GSM3239808 WT T Pre-transfer rep 3
BioProject PRJNA478772
SRA SRP151712

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Supplementary file Size Download File type/resource
GSE116475_2_vs_1.deseq.csv.gz 734.3 Kb (ftp)(http) CSV
GSE116475_Cuff_Gene_Counts.csv.gz 796.0 Kb (ftp)(http) CSV
GSE116475_KO_v_WT.deseq.csv.gz 785.6 Kb (ftp)(http) CSV
GSE116475_STAR_Gene_Counts.csv.gz 427.6 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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