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Series GSE116691 Query DataSets for GSE116691
Status Public on Feb 08, 2019
Title Parallel analysis of RNA ends (PARE) sequencing of Barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei; isolate 5874)
Organisms Hordeum vulgare; Blumeria hordei
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012. Molecular Plant Pathology 13 (4): 414-430. DOI: 10.1111/j.1364-3703.2011.00783.x). Blumeria graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to identify small RNA-derived transcript cleavage sites from both barley and Bgh that regulate gene expression at the post-transcriptional level both within species and cross-kingdom.
 
Overall design 6 timepoints (0, 16, 20 24, 32, and 48 hours after inoculation with Bgh 5874) pooled for each of 5 genotypes.

This experiment used the identical split-plot design, tissue, and source RNA as GEO submission GSE101304.
 
Contributor(s) Hunt M, Fuerst G, Wise RP
Citation(s) 31345162
Submission date Jul 05, 2018
Last update date Aug 06, 2019
Contact name Gregory Fuerst
E-mail(s) gregory.fuerst@usda.gov
Organization name USDA/ARS
Department CICGRU
Street address Department of Plant Pathology and Microbiology, Iowa State University, 1344 Advanced Teaching & Research Bldg., 2213 Pammel Dr.
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL23699 Illumina HiSeq 2500 (Blumeria graminis f. sp. hordei; Hordeum vulgare)
Samples (5)
GSM3258525 WT_pool
GSM3258526 mla6_pool
GSM3258527 rar3_pool
Relations
BioProject PRJNA479878
SRA SRP152349

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE116691_Exp21729_Lib_Info.pdf 489.7 Kb (ftp)(http) PDF
GSE116691_RAW.tar 415.9 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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