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Series GSE116703 Query DataSets for GSE116703
Status Public on Nov 28, 2018
Title RiboTag translatome analysis of outer hair cell postnatal development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In order to determine the regulators of outer hair cell postnatal maturation, we utilized the RiboTag mouse model to perform a detailed transcriptomic analysis of outer hair cells at five postnatal developmental time points: P8, P14, P28, 6 weeks (6wk) and 10 weeks (10wk). This analysis resulted in consistent enrichment of outer hair cell expressed genes in the immunoprecipitated RNA compared to whole cochlear input RNA from each time point. Using transcription factor binding motif prediction on a set of defined outer hair cell enriched genes, we further use this dataset to identify the helios transcription factor as a regulator of the postnatal outer hair cell transcriptome.
Overall design Examination of the outer hair cell translatome by outer hair cell expressed HA-tagged ribosomal immunoprecipitation at 5 postnatal timepoints (P8, P14, P21, 6wk and 10wk). Immunoprecipitated samples were compared to input cochlear RNA controls in independent biological duplicates or triplicates.
Contributor(s) Matern MS, Milon B, Hertzano R, Elkon R, Song Y
Citation(s) 30464345
Submission date Jul 06, 2018
Last update date Mar 19, 2019
Contact name yang song
Organization name University of Maryland in Baltimore
Department Department: Institute for Genome Sciences
Street address HSF-3, 3rd Floor; 670 W Baltimore St.
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (22)
GSM3258652 10w-1_input
GSM3258653 10w-1_IP
GSM3258654 10w-2_input
BioProject PRJNA479956
SRA SRP152410

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE116703_RAW.tar 5.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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