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| Status |
Public on Jul 12, 2018 |
| Title |
Zearalenone exposure enhanced the expression of tumorigenesis genes in donkey granulosa cells via the PTEN/PI3K/AKT signaling pathway |
| Organism |
Equus asinus africanus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigated the biological effects of ZEA exposure on donkey granulosa cells by using RNA-seq analysis. ZEA at 10 and 30 μM were administered to granulosa cells within 72 hours of in vitro culture. ZEA at 10 μM significantly altered the tumorigenesis associated genes in donkey granulosa cells. Exposure to 10 and 30 μM ZEA treatment significantly reduced mRNA expression of PTEN, TGFβ, ATM, and CDK2 genes, particularly, the ZEA treatment significantly increased the expression of PI3K and AKT genes. Furthermore, immunofluorescence, RT-qPCR, and Western blot analysis verified the gene expression of ZEA-exposed granulosa cells. Collectively, these results demonstrated the deleterious effect of ZEA exposure on the induction of ovarian cancer related genes via the PTEN/PI3K/AKT signaling pathway in donkey granulosa cells in vitro.
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| Overall design |
9 mRNA profiles of donkey granulosa cells were generated by RNA sequencing.
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| Contributor(s) |
Zhang RQ, Zhang GL |
| Citation(s) |
30108608 |
| |
| Submission date |
Jul 11, 2018 |
| Last update date |
May 17, 2019 |
| Contact name |
Zhang Rui Qian |
| E-mail(s) |
zhangruiqianv@gmail.com
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| Organization name |
Qingdao Agricultural University
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| Street address |
Great Wall Road
|
| City |
QingDao |
| State/province |
ShanDong |
| ZIP/Postal code |
266109 |
| Country |
China |
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| Platforms (1) |
| GPL25320 |
Illumina HiSeq 2000 (Equus asinus africanus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA480694 |
| SRA |
SRP153098 |
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