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Series GSE117052 Query DataSets for GSE117052
Status Public on Jul 01, 2020
Title Transcriptome analysis of apoplastic reactive oxygen species signaling in Arabidopsis thaliana accessions with varying ozone sensitivity.
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Ozone (O3) is a phytotoxic air pollutant that enters the plant through stomata and activates cell death programs leading to development of lesions in sensitive plant species. Exposure to O3 provokes the formation of reactive oxygen species (ROS) in the apoplast of plant cells. Imbalanced ROS homeostasis has deleterious toxic effects on DNA, proteins, lipids, and carbohydrates. However, ROS are not merely damaging molecules, as they also initiate signaling events that help plants acclimate to stress. Like under most abiotic and biotic stresses, apoplastic ROS signaling triggered by O3 induces massive changes in gene expression, enzyme activities and metabolic profiles. Thus, O3 is a very useful tool to study general mechanisms of ROS signaling and regulation of gene expression. Here we used a combination of transcriptome analysis and cell death assays to identify molecular mechanism initiated by apoplastic ROS signaling involved in the regulation of defense signaling and cell death in Arabidopsis thaliana.
Overall design Transcriptome profiling of ozone response in three Arabidopsis accessions with different ozone sensitivity (Col-0, Shahdara and Cvi-0) to identify molecular mechanisms triggered by apoplastic ROS signaling. Raw sequencing data for Col-0 was previously submitted (GSE65740) and re-analyzed in the current study.
Contributor(s) Morales LO, Leppälä J, Safronov O, Brosché M
Citation(s) 33624812
Submission date Jul 12, 2018
Last update date Jun 03, 2021
Contact name Luis Morales
Phone +46 19 301033
Organization name Örebro University
Department School of Science and Technology
Street address Fakultetsgatan 1
City Örebro
ZIP/Postal code SE-70182
Country Sweden
Platforms (2)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (12)
GSM3267806 Shahdara.control_I
GSM3267807 Shahdara.control_II
GSM3267808 Shahdara.control_III
Reanalysis of GSM1603940
Reanalysis of GSM1603941
Reanalysis of GSM1603942
Reanalysis of GSM1603943
Reanalysis of GSM1603944
Reanalysis of GSM1603945
BioProject PRJNA480911
SRA SRP153258

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Supplementary file Size Download File type/resource
GSE117052_gene_counts.txt.gz 2.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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