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| Status |
Public on Jul 01, 2020 |
| Title |
Transcriptome analysis of apoplastic reactive oxygen species signaling in Arabidopsis thaliana accessions with varying ozone sensitivity. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ozone (O3) is a phytotoxic air pollutant that enters the plant through stomata and activates cell death programs leading to development of lesions in sensitive plant species. Exposure to O3 provokes the formation of reactive oxygen species (ROS) in the apoplast of plant cells. Imbalanced ROS homeostasis has deleterious toxic effects on DNA, proteins, lipids, and carbohydrates. However, ROS are not merely damaging molecules, as they also initiate signaling events that help plants acclimate to stress. Like under most abiotic and biotic stresses, apoplastic ROS signaling triggered by O3 induces massive changes in gene expression, enzyme activities and metabolic profiles. Thus, O3 is a very useful tool to study general mechanisms of ROS signaling and regulation of gene expression. Here we used a combination of transcriptome analysis and cell death assays to identify molecular mechanism initiated by apoplastic ROS signaling involved in the regulation of defense signaling and cell death in Arabidopsis thaliana.
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| Overall design |
Transcriptome profiling of ozone response in three Arabidopsis accessions with different ozone sensitivity (Col-0, Shahdara and Cvi-0) to identify molecular mechanisms triggered by apoplastic ROS signaling. Raw sequencing data for Col-0 was previously submitted (GSE65740) and re-analyzed in the current study.
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| Contributor(s) |
Morales LO, Leppälä J, Safronov O, Brosché M |
| Citation(s) |
33624812 |
| |
| Submission date |
Jul 12, 2018 |
| Last update date |
Jun 03, 2021 |
| Contact name |
Luis Morales |
| E-mail(s) |
luis.morales@oru.se
|
| Phone |
+46 19 301033
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| Organization name |
Örebro University
|
| Department |
School of Science and Technology
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| Street address |
Fakultetsgatan 1
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| City |
Örebro |
| ZIP/Postal code |
SE-70182 |
| Country |
Sweden |
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| Platforms (2) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| Reanalysis of |
GSM1603940 |
| Reanalysis of |
GSM1603941 |
| Reanalysis of |
GSM1603942 |
| Reanalysis of |
GSM1603943 |
| Reanalysis of |
GSM1603944 |
| Reanalysis of |
GSM1603945 |
| BioProject |
PRJNA480911 |
| SRA |
SRP153258 |
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