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Series GSE117358 Query DataSets for GSE117358
Status Public on Jul 18, 2019
Title RNA-sequencing of pretreatment AB1 (mesothelioma) and Renca (kidney cancer) tumours mice both respondant and non-respondant to immunotherapy with CTLA4 and PD-L1
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Cancer immunotherapy has shown outstanding results in the past few years. Specifically, antibodies targeting immune checkpoints can prolong survival in some patients with various cancer types. However, most patients do not respond, and it is not fully understood what biological processes determine an effective outcome. This lack of understanding hinders the development of rational combination treatments.
Methods: Here, we analysed bulk RNAseq data obtained from pretreatment responding and non-responding tumour samples from mice treated with antibodies against CTLA4 and PD-L1.
Results: We found that responsive tumours displayed an inflammatory gene expression signature which was consistent with positive upstream regulation by not only IFNγ/STAT1, but also TLR3 and negative regulation by IL-10. Our results identify a pre-treatment cellular microenvironment and molecular signature associated with benefit from immune checkpoint blockade that can be therapeutically attained to improve treatment efficacy.
Overall design 48 samples in total: 24 samples from AB1 pretreatment tumours (12 responders, 12 non-responders) and 24 samples from Renca pretreatment tumours (12 responders, 12 nonresponders). Each sample represents an individual biological replicate (i.e. no duplicate samples from the same mouse). Response to immunotherapy determined….
Contributor(s) Zemek RM, de Jong E, Chin WL, Fear V, Casey T, Forbes C, Hope D, Boon L, Forrest AR, Muiri DO, Millward MJ, Nowak AK, Lassmann T, Bosco A, Lake RA, Lesterhuis WJ
Citation(s) 31316010
Submission date Jul 19, 2018
Last update date Nov 17, 2020
Contact name Emma de Jong
Organization name Telethon Kids Institute
Street address 15 Hospital Ave
City Nedlands, Perth
State/province WA
ZIP/Postal code 6009
Country Australia
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (48)
GSM3291713 AB1 Responder rep1 [AB01]
GSM3291714 AB1 Non-responder rep1 [AB02]
GSM3291715 AB1 Responder rep2 [AB09]
BioProject PRJNA481876
SRA SRP154425

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Supplementary file Size Download File type/resource
GSE117358_genecounts.csv.gz 2.5 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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