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Series GSE117365 Query DataSets for GSE117365
Status Public on Feb 27, 2019
Title New and subgroup-specific miRNAs and miRNA-offset RNA unraveled by RNA-sequencing in multiple myeloma [miRNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Microarray analysis of the miRNome in multiple myeloma (MM) has unraveled the differential expression of miRNAs in cytogenetic subgroups, their involvement in the tumor biology and their effectiveness in prognostic models. Herein, we provide a view of small RNA transcriptional landscape in MM at a higher definition, exploiting the possibilities offered by small RNA-sequencing (sRNA-seq), including improved detection of known/new mature species and accurate investigation of isomiRs and miRNA-offset RNAs (moRNAs). Matched sRNA-seq data and miRNA microarray profiles in a panel of 30 primary MM tumors were obtained and compared. A good concordance was observed in the detection of the expression level of known species and in the differential analysis between subgroups. The superior discovery power of RNA-seq allowed characterizing 655 known and 17 new mature miRNAs, and describing 74 moRNAs, most of which highly expressed and group-specific. Ectopic expression of miR-135 in t(4;14) patients, undetected by arrays was detected by RNA-seq and validated by qPCR.
 
Overall design Total RNA samples from highly purified plasma cells of 30 MM cases at onset
 
Contributor(s) Agnelli L, Bisognin A, Gaffo E, Todoerti K, Manzoni M, Galletti S, Bortoluzzi S, Neri A
Citation(s) 30783080
Submission date Jul 19, 2018
Last update date Mar 27, 2019
Contact name Luca Agnelli
E-mail(s) luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
Phone +390223903581
Organization name IRCCS Istituto Nazionale dei Tumori
Department Department of Advanced Diagnostics
Street address Venezian 1
City MILAN
ZIP/Postal code 20133
Country Italy
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (30)
GSM3291833 MM at diagnosis, sample 1 [miRNA-Seq]
GSM3291834 MM at diagnosis, sample 2 [miRNA-Seq]
GSM3291835 MM at diagnosis, sample 3 [miRNA-Seq]
This SubSeries is part of SuperSeries:
GSE117366 New and subgroup-specific miRNAs and miRNA-offset RNA unraveled by RNA-sequencing in multiple myeloma
Relations
BioProject PRJNA481916
SRA SRP154444

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117365_raw_data.txt.gz 27.0 Kb (ftp)(http) TXT
GSE117365_raw_variants.txt.gz 384.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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