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| Status |
Public on May 17, 2019 |
| Title |
Differential expression data between E15.5 outflow tracts of Gata4 G295S mutant embryos and littermate controls |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
E15.5 embryos were micro-disscted from Gata4 G295S mutant mice and littermate controls, RNA was isolated using Norgen total RNA isolation, and libraries were generated with the RNA TruSeq Stranded Total RNA kit. 50 base pair paired end reads were obtained on an illumina high seq 2500. Fastq files were aligned to the mouse genome using STAR aligner. QC was performed using RNASeQC and RSeQC. BAM files were processed using cufflinks pipeline.
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| Overall design |
The project aims to assess the differential gene expression at E15.5 between the outflow tracts of Gata4 G295S mutant embryos and wildtype littermate controls.
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| Contributor(s) |
LaHaye S, Garg V |
| Citation(s) |
31138536 |
| |
| Submission date |
Jul 24, 2018 |
| Last update date |
Jul 09, 2019 |
| Contact name |
Vidu Garg |
| E-mail(s) |
vidu.garg@nationwidechildrens.org
|
| Phone |
614-355-5710
|
| Organization name |
Research Institute at Nationwide Children's Hospital
|
| Department |
Cardiovascular Research
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| Lab |
Vidu Garg
|
| Street address |
700 Children's Drive WB4239
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43205 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA482668 |
| SRA |
SRP155044 |
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