|
| Status |
Public on Dec 22, 2008 |
| Title |
Nude mouse model of endometriosis |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The pathophysiology of endometriotic lesion development remains unclear but involves a complex interaction between ectopic endometrium and host peritoneal tissues. We hypothesised that disruption of this interaction was likely to suppress endometriotic lesion formation. We hoped to delineate the molecular and cellular dialogue between ectopic human endometrium and peritoneal tissues in nude mice, as a first step towards testing this hypothesis. Human endometrium was xenografted into nude mice and the resulting lesions were analysed using microarrays. A novel technique was developed that unambiguously determined whether RNA transcripts identified by the microarray analyses originated from human cells (endometrium) or mouse cells (stroma). Four key pathways (ubiquitin/proteosome, inflammation, tissue remodelling/repair and ras-mediated oncogenesis) were revealed, that demonstrated communication between host stromal cells and ectopic endometrium.
Morphometric analysis of nude mouse lesions confirmed that necrosis, inflammation, healing and repair and cell proliferation occurred during xenograft development. These processes were entirely consistent with the molecular networks revealed by the microarray data. The transcripts detected in the xenografts overlapped with transcripts differentially expressed in a comparison between paired eutopic and ectopic endometrium from human endometriotic patients.
For the first time components of the interaction between ectopic endometrium and peritoneal stromal tissues have been revealed in ectopic endometrial lesions. Targeted disruption of this dialogue is likely to disrupt endometriotic tissue formation and may prove to be an effective therapeutic strategy for endometriosis.
Keywords: time course, disease state analysis.
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| Overall design |
Xenografts of human endometrium in nude mice hybridised to both human and mouse specific genechips. 2 biological replicates, at 3 time points. Control human endometrium and mouse pertioneum were hybridised to both human and mouse genechips
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| |
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| Contributor(s) |
Charnock-Jones DS |
| Citation(s) |
18688027 |
| |
| Submission date |
Jun 12, 2008 |
| Last update date |
Dec 13, 2018 |
| Contact name |
D. Stephen Charnock-Jones |
| E-mail(s) |
dscj1@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Obstetrics and Gynaecology
|
| Street address |
The Rosie Hospital
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0SW |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
|
| Samples (18)
|
| GSM298019 |
mouse peritoneum time 0 mouse array replicate 1 |
| GSM298020 |
mouse peritoneum time 0 human array replicate 1 |
| GSM298021 |
human endometrium time 0 human array replicate 1 |
| GSM298022 |
human endometrium time 0 human array replicate 2 |
| GSM298023 |
human endometrium time 0 mouse array replicate 1 |
| GSM298024 |
human endometrium time 0 mouse array replicate 2 |
| GSM298025 |
xenograft 7 day mouse array replicate 1 |
| GSM298026 |
xenograft 7 day mouse array replicate 2 |
| GSM298027 |
xenograft 10 day mouse array replicate 1 |
| GSM298028 |
xenograft 10 day mouse array replicate 2 |
| GSM298029 |
xenograft 14 day mouse array replicate 1 |
| GSM298030 |
xenograft 14 day mouse array replicate 2 |
| GSM298031 |
xenograft 7 day human array replicate 1 |
| GSM298032 |
xenograft 7 day human array replicate 2 |
| GSM298033 |
xenograft 10 day human array replicate 1 |
| GSM298034 |
xenograft 10 day human array replicate 2 |
| GSM298035 |
xenograft 14 day human array replicate 1 |
| GSM298036 |
xenograft 14 day human array replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE11769 |
Analysis of ectopic human endometrium and peritoneal tissues in nude mice |
|
| Relations |
| BioProject |
PRJNA109079 |
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