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Series GSE117783 Query DataSets for GSE117783
Status Public on Feb 28, 2019
Title Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 1]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The weekly turnover of the intestinal epithelium is driven by multipotent, Lgr5+, crypt base columnar cells (CBCs). In response to injury, however, Lgr5+ cells are lost but then re-emerge and are required for successful recovery. How these resurgent Lgr5+ stem cells arise is unclear. We transcriptionally profiled single cells from regenerating intestinal epithelia and identified a unique cell type we term the revival stem cell (rSC). rSCs are mutually exclusive to CBCs and are distinguished by elevated expression of cell survival and DNA repair genes. In homeostasis, rSCs are extremely rare, but nevertheless give rise to all the major cell types of the intestine including crypt-villus axes. After damage rSCs display a 20-fold, Yap-dependent, transient expansion, reconstitute the Lgr5+ CBC compartment and are required to regenerate a functional intestine. These studies define a unique stem cell phenotype that is mobilized by damage to reconstitute the intestinal epithelium.
 
Overall design Examination of regenerating mouse intestinal epithelium
 
Contributor(s) Ayyaz A, Kumar S, Sangiorgi B, Ghoshal B, Trcka D, Wrana JL, Gregorieff A
Citation(s) 31019301, 32656539
Submission date Jul 27, 2018
Last update date Jul 15, 2020
Contact name Bibaswan Ghoshal
E-mail(s) bibaswan.ghoshal@gmail.com
Organization name Lunenfeld Tanenbaum Research Institute
Street address 600 University Avenue
City Toronto
ZIP/Postal code M5G 1X5
Country Canada
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM3308717 Whole Epithelium-Normal
GSM3308718 Crypts-Normal
GSM3308719 Whole Epithelium-Irradiated
This SubSeries is part of SuperSeries:
GSE123516 Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell
Relations
BioProject PRJNA483187
SRA SRP155511

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117783_Crypts_processed.csv.gz 162.1 Mb (ftp)(http) CSV
GSE117783_RAW.tar 112.7 Mb (http)(custom) TAR (of MTX, TSV)
GSE117783_Whole_epithelium_processed.csv.gz 146.7 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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