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Series GSE118063 Query DataSets for GSE118063
Status Public on Nov 15, 2018
Title Multivalent H3K9me2 Reader ADCP1 Functions as a plant HP1 to Maintain Epigenetic Silencing
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Heterochromatin formation is important for genome stability and transposon silencing. The evolutionally conserved Heterochromatin Protein 1 (HP1) recognizes H3K9me3 and maintains heterochromatin from fission yeast to mammalians. However, it is still questioned about the presence of HP1 protein in plants. Here, we discovered a multivalent H3K9me2 reader ADCP1 in Arabidopsis through biophysical and structural studies, and agenet domain in ADCP1 has the similar function of chromo domain in HP1. We further showed that ADCP1 was essential for heterochromatin maintenance through modulating H3K9me2 and DNA methylation levels. Similar to human HP1alpha and fly HP1a, ADCP1 was also able to mediate heterochromatin phase separation both in vitro and in vivo. Our results demonstrate the plant specific and evolution conserved ADCP1 functions as the HP1 equivalent protein in plant.
 
Overall design ADCP1 ChIP performed with GFP antibody using ADCP1-GFP tagged transgenic plants, and Col-0 with no tag as control. H3K9me2 ChIP performed in Col-0 and adcp1-1 mutant and H3 ChIP as positive control. BS-seq perfomed with gDNA of Col-0 and adcp1-1. Total RNAs extracted from Col-0 and adcp1-1 for sequencing.
 
Contributor(s) Cheng L, Sun Q
Citation(s) 30425322
Submission date Aug 02, 2018
Last update date Jan 02, 2019
Contact name QIANWEN SUN
E-mail(s) sunqianwen@tsinghua.edu.cn
Organization name Tsinghua University
Department School of life sciences
Street address Zhongguancun Street
City Beijing
ZIP/Postal code 100084
Country China
 
Platforms (1)
GPL23157 HiSeq X Ten (Arabidopsis thaliana)
Samples (22)
GSM3317914 Col-0_H3K9me2_ChIPSeq_rep1
GSM3317915 Col-0_H3_ChIPSeq_rep1
GSM3317916 adcp1-1_H3K9me2_ChIPSeq_rep1
Relations
BioProject PRJNA484219
SRA SRP156229

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Supplementary file Size Download File type/resource
GSE118063_RAW.tar 1.7 Gb (http)(custom) TAR (of BW, GTF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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