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Series GSE119169 Query DataSets for GSE119169
Status Public on Jan 31, 2019
Title Rbpj expression in regulatory T cells is critical for restraining TH2 responses
Organism Mus musculus
Experiment type Expression profiling by array
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary This SuperSeries is composed of the SubSeries listed below.

The transcriptional regulator Rbpj is involved in T-helper (TH) subset polarization, but its function in Treg cells remains unclear. Here we show that Treg-specific Rbpj deletion leads to splenomegaly and lymphadenopathy despite increased numbers of Treg cells with a polyclonal TCR repertoire. A specific defect of Rbpj-deficient Treg cells in controlling TH2 polarization and B cell responses is observed, leading to the spontaneous formation of germinal centers and a TH2-associated immunoglobulin class switch. The observed phenotype is environment-dependent and can be induced by infection with parasitic nematodes. Rbpj-deficient Treg cells adopt open chromatin landscapes and gene expression profiles reminiscent of tissue-derived TH2-polarized Treg cells, with a prevailing footprint of the transcription factor Gata-3. Taken together, our study suggest that Treg cells require Rbpj to specifically restrain TH2 responses, including their own excessive TH2-like differentiation potential.
 
Overall design Refer to individual Series
 
Citation(s) 30962454
Submission date Aug 28, 2018
Last update date Apr 17, 2019
Contact name Michael Delacher
E-mail(s) delacher@uni-mainz.de
Phone +49 6131 17 6574
Organization name University Medical Center of the Johannes Gutenberg-University Mainz
Department Institute for Immunology
Lab Immunology
Street address Langenbeckstrasse 1
City Mainz
State/province Rhineland-Palatinate
ZIP/Postal code 55131
Country Germany
 
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17543 Illumina MouseWG-6 v2.0 R2 expression beadchip
Samples (59)
GSM3356584 Spleen tisTreg2 R1
GSM3356585 Spleen tisTreg2 R4
GSM3356586 Spleen tisTreg2 R2
This SuperSeries is composed of the following SubSeries:
GSE119011 Rbpj expression in regulatory T cells is critical for restraining TH2 responses [spleen RbpjKO and RbpjWT RNA-seq]
GSE119111 Rbpj expression in regulatory T cells is critical for restraining TH2 responses [spleen RbpjKO sick expression]
GSE119130 Rbpj expression in regulatory T cells is critical for restraining TH2 responses [lymph nodes RbpjWT and diseased RbpjKO expression]
Relations
BioProject PRJNA488292

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119169_RAW.tar 4.2 Gb (http)(custom) TAR (of BIGWIG, IDAT)
SRA Run SelectorHelp

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