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Series GSE119506 Query DataSets for GSE119506
Status Public on Apr 25, 2019
Title Single-cell RNA-seq analysis of human tonsil CD14+ cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We performed single-cell RNA-seq on CD14+ cells isolated from the tonsils of one healthy donor. We used the 10x chromium technology.
Overall design Tonsil phagocytes were prepared by centrifugation on a Ficoll gradient. Dendritic cells and macrophages were enriched by negative selection using magnetic beads. Cell populations were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer’s protocol.
Contributor(s) Segura E, Durand M, Bohec M, Gueguen P
Citation(s) 31072818
Submission date Sep 05, 2018
Last update date Jul 25, 2019
Contact name Paul Gueguen
Organization name Institut Curie
Department Inserm U932
Street address 26 rue D'Ulm
City Paris
ZIP/Postal code 75005
Country France
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (1)
GSM3375767 tonsil CD14+ cells
BioProject PRJNA489435
SRA SRP159650

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Supplementary file Size Download File type/resource
GSE119506_RAW.tar 10.1 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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