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Series GSE120038 Query DataSets for GSE120038
Status Public on Mar 01, 2019
Title Effect of miR-92a-3p and/or miR-489-3p inhibition on aorta endothelium of Apoe-/-;5/6Nx
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The role of endothelial miR-92a-3p and miR-489-3p in CKD-associated atherosclerosis was examined by mRNA expression analysis in Apoe-/- mice with 5/6 nephrectomy treated with locked-nucleic acid (LNA) inhibitors complexed with high-density lipoprotein (HDL).
 
Overall design RNA sequencing of aortic endothelial cell total RNA from Apoe-/- (B6.129P2-Apoetm1Unc/J) with 5/6 nephrectomy for 7 weeks and one week after treatment with saline, HDL, HDL + LNA92a, HDL + LNA489, or HDL + LNA92a + LNA489. Surgery was performed on 9 week old female mice maintained on regular chow diet. The mice recieved a single retro-orbital treatment 7 weeks post surgery and were sacrificed 1 week post treatment. The aortic endothelium was sheared to collect total RNA, which was sequenced with 4 biological replicates per group.
 
Contributor(s) Vickers KC, Wiese CB
Citation(s) 30731284
Submission date Sep 17, 2018
Last update date May 31, 2019
Contact name Kasey C Vickers
E-mail(s) kasey.c.vickers@vumc.org
Organization name Vanderbilt University
Street address 2220 Pierce Ave
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (20)
GSM3391949 Saline 1
GSM3391950 Saline 2
GSM3391951 Saline 9
Relations
BioProject PRJNA491430
SRA SRP161894

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120038_RNAseq_mouse.count.vsd.txt.gz 1.8 Mb (ftp)(http) TXT
GSE120038_RNAseq_mouse.fpkm.tsv.gz 1.4 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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