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Series GSE120257 Query DataSets for GSE120257
Status Public on Jun 10, 2019
Title HDAC5AA but not HDAC5DAD regulate the expression of genes associated with axon growth
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Expressing HDAC5 mutant whose serine 259 and 488 have been replaced by alanine (HDAC5AA) promotes optic nerve regeneration in retinal ganglion cells. However, expressing GFP, HDAC5WT and HDAC5DAD, whose serine 259 and 498 have been replaced by aspartic acid and serine 280 by alanine, do not promote optic nerve regeneration. The goal of this experiment was to determine the underlying mechanisms leading to the phenotypical differences in optic nerve regeneration between control GFP, HDAC5DAD, and HDAC5AA by analyzing the retinal transcriptome of the different treatments.
Overall design GFP, HDAC5DAD, and HDAC5AA were overexpressed in C57B/6 mice retinal ganglion cells by intravitreally injecting adenoassociated virus (AAV2) containing the vector of expression for these genes. After two weeks, these animals received an optic nerve crush. On the 4th day post injury, mice were sacrificed and retinas were extracted for sequencing.
Contributor(s) Pita-Thomas W, Mahar M, Cavalli V
Citation(s) 30910408
Submission date Sep 20, 2018
Last update date Jun 10, 2019
Contact name Valeria Cavalli
Organization name Washington University in St. Louis
Department Anatomy and Neurobiology
Street address 660 S. Euclid Ave.
City Saint Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (16)
GSM3396939 Non injured retina rep1
GSM3396940 Non injured retina rep2
GSM3396941 Non injured retina rep3
BioProject PRJNA492315
SRA SRP162260

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Supplementary file Size Download File type/resource
GSE120257_RGC_GFPvUninjured.xlsx 3.7 Mb (ftp)(http) XLSX
GSE120257_RGC_HDAC5AAvUninjured.xlsx 3.9 Mb (ftp)(http) XLSX
GSE120257_RGC_HDAC5DADvUninjured.xlsx 4.2 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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