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Status |
Public on Aug 26, 2008 |
Title |
Expression profiles and ChIP on chip genomewide experiments with deltaCR2 mutant virus |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array Genome binding/occupancy profiling by genome tiling array
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Summary |
Adenovirus e1a induces quiescent human cells to divide. We found that e1a causes global relocalization of the RB-proteins (RB, p130, p107) and p300/CBP histone acetyltransferases on promoters that restricts H3K18ac to a limited set of genes to stimulate cell cycling and inhibit antiviral responses and cellular differentiation. Soon after expression, e1a binds transiently to cell cycle/growth genes, causing enrichment of p300/CBP, PCAF, H3K18ac, depletion of RB-proteins, and transcriptional activation. e1a also associates transiently with antiviral genes, causing enrichment for RB, p130, H4K16ac, increased nucleosome density, and repression. At later times, e1a and p107 bind mainly to development/differentiation genes, repressing transcription. The temporal order of e1a binding required its interactions with p300/CBP and RB-proteins. Our data uncover a defined transcriptional and epigenetic reprogramming leading to cellular transformation.
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Overall design |
Expression profiles and ChIP on chip genomewide experiments with deltaCR2 mutant virus
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Contributor(s) |
Ferrari R, Pellegrini M, Horwitz G, Xei W, Berk A, Kurdistani S |
Citation(s) |
18719284 |
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Submission date |
Jul 09, 2008 |
Last update date |
Mar 20, 2012 |
Contact name |
Siavash K Kurdistani |
E-mail(s) |
Skurdistani@mednet.ucla.edu
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Organization name |
UCLA
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Department |
Biological Chemistry
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Lab |
Kurdistani
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Street address |
615 Charles E Young Dr South
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City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
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Platforms (2) |
GPL7031 |
Agilent Whole Human Genome Oligo Microarray - custom annotation |
GPL7032 |
Human Promoter ChIP-on-chip Microarray - custom annotation |
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Samples (5)
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GSM304309 |
Expression profile 24 hours post infection for deltaCR2 e1a mutant |
GSM304361 |
ChIP on chip deltaCR2 e1a (IP infected versus INP infected) at 6 hours post infection |
GSM304362 |
ChIP on chip deltaCR2 e1a (IP infected versus INP infected) at 24 hours post infection |
GSM304365 |
ChIP on chip histone H3K9 acetylation for deltaCR2 virus (IP infected versus IP mock) at 24 hours post infection |
GSM304368 |
ChIP on chip H3K18 acetylation in deltaCR2 virus (IP infected versus IP mock) at 24 hours post infection |
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This SubSeries is part of SuperSeries: |
GSE12543 |
Profiles and ChIP on chip experiments with dl1500 virus (expressing WT small e1a), R2G and deltaCR2 mutant viruses |
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Relations |
BioProject |
PRJNA114313 |