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Series GSE120787 Query DataSets for GSE120787
Status Public on Oct 04, 2018
Title Single cell RNA-sequencing of innate lymphoid cells isolated from mouse skin layers
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Determine the layer-specific indentities and heterogeneity of innate lymphoid cells in the skin by single cell transcriptomic analysis

Methods: Innate lymphoid cells were sorted from epidermis, dermis and subcutis of adult B6 mice and subjected to droplet based single-cell captureing and RNA isolation and library preparation.

Results: 3,431, 3,356 and 1,061 innate lymphoid cells from epidermis, dermis, and subcutis were analyzed. Unsupervised clustering of the combined data set performed in Seurat identified 6 distinct ILC clusters.

Conclusions: This study reveals skin layer-specific transcriptomic signature of innate lymphoid cells
Overall design Transcriptome profiles of skin innate lymphoid cells were generated by droplet-based single cell RNA-seq
Contributor(s) Kobayashi T, Voisin B, Nagao K
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Submission date Oct 03, 2018
Last update date Feb 11, 2019
Contact name Tetsuro Kobayashi
Organization name NIAMS/NIH
Street address 9000 Rockville Pike, Bldg 10, Rm12N256
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (3)
GSM3415619 Epidermal innate lymphoid cells
GSM3415620 Dermal innate lymphoid cells
GSM3415621 Subcutaneous innate lymphoid cells
BioProject PRJNA494536
SRA SRP163240

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Supplementary file Size Download File type/resource
GSE120787_RAW.tar 22.1 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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