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Series GSE120867 Query DataSets for GSE120867
Status Public on Oct 04, 2020
Title Transcriptome profiling of wildtype, nonoperated mouse hearts compared to wildtype, sham operated hearts
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of the present study was to investigate the activation of the cardiac inflammatory response in the hearts of SHAM (surgical opening of the chest) mice in comparison to CONTROL hearts isolated from naïve (not operated) mice. Male C57BL/6J (10 weeks) were subjected to an open-chest ischemia-reperfusion (I/R) SHAM procedure in order to examine the effects of this surgery alone in producing cardiac inflammation. The procedure consisted of thoracotomy and a suture passed around the left anterior descending artery (LAD). The mouse chest was kept open for approximately 45 min. We chose to study an open-chest I/R SHAM because this is the reference surgery for the most common surgical procedure used to study cardiac inflammation. Mice were sacrificed 5 days after the procedure and the hearts were removed and used in RNAseq experiments.
Overall design mRNA sequencing profiles were generated from 5 each of SHAM (sham-operated) and CONTROL (naïve, no surgery) hearts on an Illumina HiSeq 3000 instrument.
Contributor(s) Rocha-Resende C, Matkovich SJ, Mann DL
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Submission date Oct 05, 2018
Last update date Oct 04, 2020
Contact name Scot J Matkovich
Organization name Washington University School of Medicine
Department Center for Cardiovascular Research
Street address 660 S Euclid Ave, box 8086
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (10)
GSM3417424 control (naive) 1
GSM3417425 control (naive) 2
GSM3417426 control (naive) 3
BioProject PRJNA494802
SRA SRP163584

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Supplementary file Size Download File type/resource
GSE120867_unprocessed_HTSeq_GRCm38_data.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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