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Series GSE120966 Query DataSets for GSE120966
Status Public on Aug 30, 2019
Title Single-cell RNA-seq Reveals a Developmental Hierarchy and Oncogenic Networks for Initiation of Medulloblastoma [10x Genomics Chromium]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary By utilizing single-cell analysis at different stages of tumorigenesis, we demonstrated a developmental hierarchy of dynamic progenitor pools in murine sonic hedgehog-(SHH) MBs. We identified Olig2+ progenitors as transit-amplifying cells during initial tumorigenic phases. These cells are quiescent stem-like progenitors in full-blown tumors but enriched in therapy-resistant as well as recurrent medulloblastomas. Olig2 ablation or depletion of mitotic Olig2+ progenitors abrogated tumorigenesis. Transcriptome profiling and chromatin occupancy assays revealed that Olig2 activates oncogenic networks including HIPPO- Yap/Taz signaling and Aurora-A/MycN pathways. Co-targeting these oncogenic pathways induced sustained tumor growth arrest. Together, our single-cell analyses uncover unexpected glia-lineage-related Olig2+ progenitor pools critical for medulloblastoma initiation and suggest targeting Olig2-regulated oncogenic pathways as an avenue for therapy.
 
Overall design one 10X genomics chromium sample from mice medullablastoma tumor cells
 
Contributor(s) Zhang L, He X, Lu R
Citation(s) 31474569
Submission date Oct 09, 2018
Last update date Nov 29, 2019
Contact name Richard Lu
Organization name Cincinnati Children's Hospital Medical Center
Department CBDI
Lab Lu Lab,T6.525
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (1)
GSM3423041 10x Genomic Chromium
This SubSeries is part of SuperSeries:
GSE120974 Single-cell RNA-seq Reveals a Developmental Hierarchy and Oncogenic Networks for Initiation of Medulloblastoma
Relations
BioProject PRJNA495592
SRA SRP165234

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE120966_RAW.tar 38.3 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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