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Series GSE121028 Query DataSets for GSE121028
Status Public on May 01, 2019
Title Distinct Polycomb Response Elements control chromatin state and gene activation of the vestigial gene
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Patterned expression of many developmental genes is thought to rely on chromatin-mediated repression. Regulatory DNA sequences called Polycomb Response Elements (PREs) play critical roles in repression. While PREs in transgenes can nucleate trimethylation on lysine 27 of the histone H3 tail (H3K27me3), none have been demonstrated to be necessary at endogenous chromatin domains. This is thought to be due to the fact that most endogenous H3K27me3 domains contain many PREs, and individual PREs may be redundant. We show here that PREs near the wing selector gene vestigial have distinctive roles at their endogenous locus, even though both PREs are repressors in transgenes. First, a PRE near the promoter is required for vestigial activation and not for repression. Second, only the distal PRE contributes to H3K27me3, but even removal of both PREs does not eliminate H3K27me3 across the vestigial domain. Thus, endogenous chromatin domains appear to be intrinsically marked by H3K27me3, and PREs can enhance this chromatin modification at inactive genes.
 
Overall design We dissected Drosophila larval tissues and generated chromatin profiles using Cleavage under targets and Release using nuclease (CUT&RUN), in which controlled cleavage by an antibody-tethered micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end sequencing.
 
Contributor(s) Ahmad K, Spens A
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Submission date Oct 09, 2018
Last update date May 02, 2019
Contact name Kami Ahmad
E-mail(s) kahmad@fredhutch.org
Organization name Fred Hutchinson Cancer Research Center
Street address 1100 Fairview Ave N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (13)
GSM3424758 CL1_wing_K27me3_(20170313_AS_DmSc_353_WDiC_187)
GSM3424759 CL1_wing_Pc_(20180424_KA_DmSc_878S)
GSM3424760 COT2_wing_FACS_K27me3_(20180424_KA_DmSc_909PX)
Relations
BioProject PRJNA495375
SRA SRP164757

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Supplementary file Size Download File type/resource
GSE121028_G021_disc_CUT_RUN.pdf 160.5 Kb (ftp)(http) PDF
GSE121028_RAW.tar 642.5 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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