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Series GSE121185 Query DataSets for GSE121185
Status Public on Feb 22, 2019
Title Transcriptional profile of the liver of zebrafish after feeding with 0% or 25% dietary maize substitution
Organism Danio rerio
Experiment type Expression profiling by array
Summary For the toxicological risk assessment of genetically modified (GM) food components, the European Food Safety Agency advises to use an adapted version of the 90-day oral toxicity study in rodents, originally designed for the toxicity testing of chemicals. Since in GM crop testing scenarios the crop under evaluation is specifically intended as a food / feed component, some testing aspects are conceptually different from testing chemical compounds and thus need careful consideration. One key consideration relates to the appropriate dose level to evaluate the GM crop as a component of the experimental diet. The aim of this study was to develop and optimize methods that are required for advancing the use of zebrafish feeding trials for the toxicological safety evaluation of GM crops. Using maize as a case study, we investigated the effects of an increasing maize substitution dose level in the diets of zebrafish, using non-GM maize. In addition, we investigated the effect of the presence of maize in the diets (0% or 25% of maize substitution levels) on mRNA transcriptional profiles in the zebrafish liver.
 
Overall design 0% vs 25% dietary maize substitution, 4 biological replicates, 10 male and 10 female fish per replicate
 
Contributor(s) Gabriëls IJ, Vergauwen L, De Boevre M, Van Dongen S, Blust R, De Saeger S, Eeckhout M, De Loose M, Knapen D
Citation(s) 30909578
Submission date Oct 12, 2018
Last update date May 24, 2019
Contact name Isabelle J Gabriëls
E-mail(s) isabelle.gabriels@uantwerpen.be
Organization name University of Antwerp
Street address Universiteitsplein 1
City Wilrijk
ZIP/Postal code 2610
Country Belgium
 
Platforms (1)
GPL14664 Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version)
Samples (12)
GSM3427476 ArrayA
GSM3427477 ArrayB
GSM3427478 ArrayC
Relations
BioProject PRJNA496036

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE121185_RAW.tar 69.8 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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