 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 24, 2019 |
| Title |
Dlx1/2 Are Central and Essential Components in the Transcriptional Code for Generating Olfactory Bulb Interneurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Generation of olfactory bulb (OB) interneurons requires neural stem/progenitor cell specification, proliferation, differentiation, and young interneuron migration and maturation. Here, we show that the homeobox transcription factors Dlx1/2 are central and essential components in the transcriptional code for generating OB interneurons. In Dlx1/2 constitutive null mutants, the differentiation of GSX2+ and ASCL1+ neural stem/progenitor cells in the dorsal lateral ganglionic eminence is blocked, resulting in a failure of OB interneuron generation. In Dlx1/2 conditional mutants (hGFAP-Cre; Dlx1/2F/- mice), GSX2+ and ASCL1+ neural stem/progenitor cells in the postnatal subventricular zone also fail to differentiate into OB interneurons. In contrast, overexpression of Dlx1&2 in embryonic mouse cortex led to ectopic production of OB-like interneurons that expressed Gad1, Sp8, Sp9, Arx, Pbx3, Etv1, Tshz1 and Prokr2. Pax6 mutants generate cortical ectopia with OB-like interneurons, but do not do so in compound Pax6; Dlx1/2 mutants. We propose that DLX1/2 promote OB interneuron development mainly through activating the expression of Sp8/9, which further promote Tshz1 and Prokr2 expression. Based on this study, in combination with earlier ones, we propose a transcriptional network for the process of OB interneuron development.
This SuperSeries is composed of the SubSeries listed below.
|
| |
|
| Overall design |
Refer to individual Series
|
| |
|
| Citation(s) |
30796806 |
| |
| Submission date |
Oct 14, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Guoping Liu |
| E-mail(s) |
gpliu@fudan.edu.cn
|
| Phone |
18521006300
|
| Organization name |
Fudan University
|
| Street address |
138 Yi Xue Yuan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (10)
|
|
| This SuperSeries is composed of the following SubSeries: |
| GSE121213 |
Transcription Factors Dlx1/2 Are Required for Making All Interneurons in the Olfactory Bulb [Pax6] |
| GSE121214 |
Transcription Factors Dlx1/2 Are Required for Making All Interneurons in the Olfactory Bulb [Dlx1/2] |
|
| Relations |
| BioProject |
PRJNA496339 |
|
|
|
|
 |
Less...
More...