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Series GSE121292 Query DataSets for GSE121292
Status Public on Aug 21, 2019
Title Sex chromsomes affect gene expression of autoantigen specific CD4+ T cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the role of sex chromsomes in sex differences of autoimmune disease, we used high throughput RNA sequencing to analyze the transciptomes of autoantigen specific CD4+ T cells from XX and XY- mice using the “four core genotypes” mouse model.
Overall design Female mice from the "four core genotypes" mouse model (XX and XY-) were gonadectomized at 4-6 weeks old and subsequently immunized with MOG 35-55 peptide emulsified in complete fraunds adjuvant, suppplemented with myobacterium tuberculosis H27Ra for 10 days. Auxillary, brachial, and inguinal draining lymph nodes were collected and processed into single cell suspensions. Cells were stiumulated with MOG 35-55 and IL-12 for 36 hours. Stimulated cells were sorted for CD4+ T cells using the EasySep Mouse CD4+ T cell isolation kit (STEMCELL), and RNA was isolated for analysis by high throughput RNA sequencing.
Contributor(s) Golden L, Itoh Y, Itoh N, Voskuhl R
Citation(s) 31403472, 31822606
Submission date Oct 16, 2018
Last update date Jan 08, 2020
Contact name Yuichiro Itoh
Phone 3102068999
Organization name UCLA
Department Neurology
Street address 635 Charles E. Young Drive South
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM3430954 XX_522
GSM3430955 XX_524
GSM3430956 XX_527
BioProject PRJNA496594
SRA SRP165833

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Supplementary file Size Download File type/resource
GSE121292_B6.CD4_rawcounts.xlsx 2.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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