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Series GSE121705 Query DataSets for GSE121705
Status Public on Aug 21, 2019
Title Sex chromsomes affect gene expression of autoantigen specific CD4+ T cells.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the role of sex chromsomes in sex differences of autoimmune disease, we used high throughput RNA sequencing to analyze the transciptomes of autoantigen specific CD4+ T cells from XX and XY- mice using the “four core genotypes” mouse model.
Overall design Female mice from the "four core genotypes" mouse model (XX and XY-) were gonadectomized at 4-6 weeks old and subsequently immunized with PLP 139-151 peptide emulsified in complete fraunds adjuvant, suppplemented with myobacterium tuberculosis H27Ra for 10 days. Auxillary, brachial, and inguinal draining lymph nodes were collected and processed into single cell suspensions. Cells were stiumulated with PLP 139-151 for 36 hours. Stimulated cells were sorted for CD4+ T cells using the EasySep Mouse CD4+ T cell isolation kit (STEMCELL), and RNA was isolated for analysis by high throughput RNA sequencing.
Contributor(s) Golden L, Itoh Y, Itoh N, Voskuhl R
Citation(s) 31403472
Submission date Oct 23, 2018
Last update date Aug 21, 2019
Contact name Yuichiro Itoh
Phone 3102068999
Organization name UCLA
Department Neurology
Street address 635 Charles E. Young Drive South
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (11)
GSM3444265 XX1
GSM3444266 XX3
GSM3444267 XX4
BioProject PRJNA498121
SRA SRP166659

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Supplementary file Size Download File type/resource
GSE121705_SJL.CD4_rawcounts.xlsx 2.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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