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Series GSE122072 Query DataSets for GSE122072
Status Public on Dec 04, 2018
Title Shortening the half-life of Cas9 maintains its gene editing ability and reduces neuronal toxicity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Virus-mediated expression of CRISPR/Cas9 has been actively used for genome editing in animal models of genetic disorders, including neurological diseases, but the consequences of overexpressing bacterial Cas9 in the mammalian brain remain unknown. Through RNA-seq analysis, we found that virus-mediated expression of Cas9 caused systematic changes in genes involved in neuronal functions. We also generated a short-lived version of Cas9, which maintains its genome editing capacity, but significantly alleviates neurotoxicity caused by overexpressed Cas9. Thus, modification of Cas9 by shortening its half-life would help develop CRISPR/Cas9-based therapeutic approaches for treating genetic neurological disorders.
Overall design Wild type mice were injected with adeno-associated virus into the striatum. Three weeks after viral injection, the striatal tissues were collected, and gene expression profiles were analyzed by RNA-seq.
Contributor(s) Yang S, Li S, Li X, Billingsley JM
Citation(s) 30517854
Submission date Nov 01, 2018
Last update date Feb 11, 2019
Contact name Gregory K Tharp
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (21)
GSM3454285 1_WT-1
GSM3454286 2_WT-2
GSM3454287 3_WT-3
BioProject PRJNA503401
SRA SRP167453

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Supplementary file Size Download File type/resource
GSE122072_Su_DESeq2_normalized_readcounts.txt.gz 2.3 Mb (ftp)(http) TXT
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