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Series GSE122097 Query DataSets for GSE122097
Status Public on Dec 11, 2019
Title RNA-seq data for: A conserved mito-cytoplasmic translational balance links two longevity pathways
Organisms Caenorhabditis elegans; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Slowing down mRNA translation in either the cytoplasm or the mitochondria are both conserved longevity mechanisms. Here, we found a non-interventional natural correlation of mitochondrial and cytoplasmic ribosomal proteins when looking at mouse population genetics, suggesting a mito-cytoplasmic translational balance. Additionally, inhibiting mitochondrial translation in C. elegans in turn reduced cytoplasmic translation and repressed growth pathways while upregulating stress responses at both proteome and transcriptome levels. This coordinated repression of cytoplasmic translation is dependent on the atf-5/Atf4 transcription factor and is conserved in mammalian cells upon inhibiting mitochondrial translation pharmacologically with the antibiotic doxycycline. Lastly, extending this to a mammalian setting using doxycycline-treated germ-free mice, we found repressed cytoplasmic translation and ribosomal proteins in liver. These data demonstrate that inhibiting mitochondrial translation initiates a signaling cascade leading to coordinated repression of cytoplasmic translation, unlike previously described unidirectional cyto-to-mito translational communication in yeast, which can be targeted to promote healthy aging.
 
Overall design In total 30 samples were sequenced: 18 were from C. elegans worms, 12 were from M. musculus mouse. Worm samples consisted of either (1) total RNA of whole worms, (2) polysomal RNA of whole worms, or (3) monosomal RNA of whole worms, performed in triplicate for both treatment (mrps-5 RNAi) and control (empty vector) worms. Mouse samples consisted of either (1) total RNA of liver or (2) polysomal RNA of liver, performed in triplicate for both treatment (doxycycline, 50mg/kg/d for two weeks) or control (amoxicillin, 50mg/kg/d for two weeks) in germ-free mice.
 
Contributor(s) Molenaars M, Janssens GE
Citation(s) 32084377
Submission date Nov 02, 2018
Last update date Mar 12, 2020
Contact name Georges Janssens
E-mail(s) g.e.janssens@amc.uva.nl
Organization name Amsterdam UMC
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105 AZ
Country Netherlands
 
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL22765 Illumina HiSeq 4000 (Caenorhabditis elegans)
Samples (30)
GSM3454838 01HT115s1
GSM3454839 02HT115s2
GSM3454840 03HT115s3
Relations
BioProject PRJNA503572
SRA SRP167691

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Supplementary file Size Download File type/resource
GSE122097_processed_data_mouse.csv.gz 1.4 Mb (ftp)(http) CSV
GSE122097_processed_data_worms.csv.gz 1.7 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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