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| Status |
Public on Dec 01, 2020 |
| Title |
Examination of Glis3 binding and affects on gene expression in mouse pancreas |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
RNA sequencing of mouse islets from mice containing a pancreas-specific deletion of Glis3, and control littermates. Also, ChIP-seq analysis of Glis3 binding using a Glis3-eGFP mouse (endogenous Glis3 fused to enhanced GFP) and GFP antibody.
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| |
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| Overall design |
Isolated Islets from 4 WT and 3 KO mice for RNAseq. Isolated islets from Glis3-eGFP mice, ChIP-seq using commercial GFP antibody
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| |
|
| Contributor(s) |
Scoville DW, Jetton AM, Grimm SA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 02, 2018 |
| Last update date |
Dec 04, 2020 |
| Contact name |
Anton M Jetten |
| E-mail(s) |
jetten@niehs.nih.gov
|
| Phone |
984-287-4094
|
| Organization name |
NIEHS, NIH
|
| Department |
DIR
|
| Lab |
Cell Biology
|
| Street address |
111 TW Alexander Drive
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (9)
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| Relations |
| BioProject |
PRJNA503637 |
| SRA |
SRP167720 |
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