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| Status |
Public on Jul 29, 2008 |
| Title |
Identification of transformation-related pathways in a breast epithelial cell model using a ribonomics approach. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Employing MCT-1 oncogene mediated transformation of immortalized breast epithelial MCF10A cells; we characterized the largely reciprocal association of these two RBPs with target mRNAs and their influence on protein expression vis-à-vis cellular transformation. Using a ribonomics approach, we identified mRNAs from cancer-related pathways whose association with AUF1 and/or HuR were altered when comparing immortalized with transformed MCF10A cells.
Significantly, we were able to demonstrate that knockdown of HuR expression using RNA interference, reduced anchorage-independent growth capacity in transformed MCF10A cells as well as decreased protein expression of a number of validated target genes. Our data demonstrate that the global alterations in binding of HuR and AUF1 with target transcripts have a critical role in post-transcriptional regulation of genes encoding proteins involved in breast epithelial cell transformation.
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| Overall design |
In this study, using a microarray approach we demonstrate that the dynamic global changes in association of two RBPs, HuR and AUF1, with cancer-related mRNAs have important influence on cell transformation in a MCT-1-mediated breast epithelial transformation model.
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| Contributor(s) |
Mazan-Mamczarz K, Hagner PR, Dai B, Wood III WH, Zhang Y, Becker KG, Liu Z, Gartenhaus RB |
| Citation(s) |
18829526 |
| |
| Submission date |
Jul 23, 2008 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Supriyo De |
| Organization name |
NIA-IRP, NIH
|
| Department |
Laboratory of Genetics and Genomics
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| Lab |
Computational Biology & Genomics Core
|
| Street address |
251 Bayview Blvd
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL6102 |
Illumina human-6 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA113731 |
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