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| Status |
Public on Nov 05, 2023 |
| Title |
Transcriptomic analysis of SLAN+ vs SLAN- non-classical monocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Monocytes can be classified into four different subsets: classic, intermediate and SLAN- and SLAN+ non-classical monocytes. SLAN+ monocytes are significantly reduced in MM patients compare to the frequency found in healthy adults (HA). Thus, we sought to analyze the transcriptome of SLAN- and SLAN+ cells to unveil the differences among both populations
We used microarrays to detail the global programme of gene expression underlying the differences among both cell subsets
We developed a 12-color combination of mAbs to evaluate the contribution of each individual marker for identifying and subsetting BM TAMs in patients with MM (N = 5): CD36-UV405, CD16-UV525, CD163-BV450, CD45-BV525, CD206-BV610, CD86-BV660, CD62L-BV763, cyCD68-FITC, SLAN-PE, HLADR-PerCPCy5.5, CD300e-APC, CD14-APCH7. This panel allowed us to identify the aforementioned four monocyte subsets. Intermediate and non-classical monocytes from healthy donors were FACS sorted based on the 7-color mAb combination - HLADR-PacB, CD45-OC515, CD36-FITC, SLAN-PE, CD16-PECy7, CD300e-APC, CD14APCH7 -
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| Overall design |
Gene expression data from CD36+ SLAN- monocytes, CD36- SLAN- monocytes, and CD36- SLAN+ monocytes.
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| Contributor(s) |
Zabaleta A, Paiva B |
| Citation missing |
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| Submission date |
Nov 05, 2018 |
| Last update date |
Nov 05, 2023 |
| Contact name |
Aintzane Zabaleta |
| E-mail(s) |
azabaletaa@unav.es
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| Organization name |
FOUNDATION FOR APPLIED MEDICAL RESEARCH
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| Street address |
Avda. Pío XII 55
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| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA503867 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE122171_RAW.tar |
73.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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