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Series GSE122359 Query DataSets for GSE122359
Status Public on Nov 09, 2019
Title β-cell adaptation in mice expressing exclusively lamin C counteracts glucose intolerance associated with aging, obesity and diabetes (LamineA-C_RNASeq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Lamin A, lamin C and progerin, produced by alternative RNA processing of LMNA gene, are intermediate filaments involved in nuclear and chromatin architecture. We investigate the ability of LMNALCS/LCS mice to adapt insulin level to physiological and physiopathologic conditions. The metabolic status of young vs old mice shows that old LMNALCS/LCS mice are obese but glucose tolerant due to an increased β cell mass and insulin secretion. By combining RNAseq and RRBS analysis, we observed a transcriptional disregulation of genes related to translation and mitochondria, confirmed by functionnal analysis, suggesting that LMNALCS/LCS mice set up a transcriptional program to adapt β cell mass and function to insulin demand. To investigate this point, we challenged young mice by inducing T1DM and T2DM. In these contexts, we show that LMNALCS/LCS mice are able to normalize their fasting glycemia by both increasing insulin secretion and regenerating β cells.
Overall design islets of Langherans isolated from wt and lmna LCS mice are analysed as 3 replicates. Each replicate is made of a pool of islets from 3 different mice
Contributor(s) de Toledo M, Chavey C, Tazi J, Pratlong M, Barrachina C
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Submission date Nov 09, 2018
Last update date Nov 09, 2019
Contact name Marion de Toledo
Organization name CNRS UMR5535
Department IGMM
Street address 1919 route de Mende
City Montpellier
ZIP/Postal code 34293
Country France
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM3464555 R2070KIKI
GSM3464556 R2170KIKI
GSM3464557 R2270KIKI
This SubSeries is part of SuperSeries:
GSE122361 β-cell adaptation in mice expressing exclusively lamin C counteracts glucose intolerance associated with aging, obesity and diabetes
BioProject PRJNA504769
SRA SRP168346

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122359_Raw_Counts_RNA-Seq_LamineA-C.txt.gz 332.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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