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Status |
Public on Nov 09, 2019 |
Title |
β-cell adaptation in mice expressing exclusively lamin C counteracts glucose intolerance associated with aging, obesity and diabetes (LamineA-C_RNASeq) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Lamin A, lamin C and progerin, produced by alternative RNA processing of LMNA gene, are intermediate filaments involved in nuclear and chromatin architecture. We investigate the ability of LMNALCS/LCS mice to adapt insulin level to physiological and physiopathologic conditions. The metabolic status of young vs old mice shows that old LMNALCS/LCS mice are obese but glucose tolerant due to an increased β cell mass and insulin secretion. By combining RNAseq and RRBS analysis, we observed a transcriptional disregulation of genes related to translation and mitochondria, confirmed by functionnal analysis, suggesting that LMNALCS/LCS mice set up a transcriptional program to adapt β cell mass and function to insulin demand. To investigate this point, we challenged young mice by inducing T1DM and T2DM. In these contexts, we show that LMNALCS/LCS mice are able to normalize their fasting glycemia by both increasing insulin secretion and regenerating β cells.
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Overall design |
islets of Langherans isolated from wt and lmna LCS mice are analysed as 3 replicates. Each replicate is made of a pool of islets from 3 different mice
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Contributor(s) |
de Toledo M, Chavey C, Tazi J, Pratlong M, Barrachina C |
Citation missing |
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Submission date |
Nov 09, 2018 |
Last update date |
Nov 09, 2019 |
Contact name |
Marion de Toledo |
E-mail(s) |
marion.detoledo@igmm.cnrs.fr
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Organization name |
CNRS UMR5535
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Department |
IGMM
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Street address |
1919 route de Mende
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City |
Montpellier |
ZIP/Postal code |
34293 |
Country |
France |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE122361 |
β-cell adaptation in mice expressing exclusively lamin C counteracts glucose intolerance associated with aging, obesity and diabetes |
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Relations |
BioProject |
PRJNA504769 |
SRA |
SRP168346 |