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Series GSE122591 Query DataSets for GSE122591
Status Public on Nov 16, 2018
Title RNA sequencing of total RNA from the vagina of hormone treated C57Bl/6J mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study was to compare transcriptome profiles (RNA seq) from the vaginas of uninfected mice. The mice have had ovariectomy surgeries and hormone replacement (estradiol, progesterone, vehicle, or a combination of estradiol and progesterone).

Methods: 6 week old female C57Bl/6J mice had ovariectomy surgeries and a week later, started hormone replacement treatment as mentioned above. After a week of hormones, the vaginas of all mice were removed and total RNA was extracted. RNA samples were sent for RNA sequencing.

Results: Upon analysis, vehicle and progesterone treated mice had similar profiles. The progesterone treated group also had progesterone stimulated genes upregulated. Estradiol regulated genes were upregulated in the estradiol treated group and the estradiol and progesterone group had a mixture of both progesterone and estradiol stimulated genes upregulated.

Conclusion: This data provided a data to ensure that our hormone replacement treatments were working by the expression of certain genes based on treatment.
 
Overall design RNA sequencing of total RNA from the vagina of hormone treated C57Bl/6J mice
 
Contributor(s) Caine EA, Diamond MS, Artyomov M, Zaitsev K
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 15, 2018
Last update date Feb 11, 2019
Contact name Maxim Artyomov
E-mail(s) martyomov@wustl.edu
Organization name WASHINGTON UNIVERSITY IN ST LOUIS
Department Pathology & Immunology
Lab Artyomov lab
Street address 425 S Euclid Ave
City St. Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (20)
GSM3475438 Vehicle replicate 1
GSM3475439 Vehicle replicate 2
GSM3475440 Vehicle replicate 3
Relations
BioProject PRJNA505685
SRA SRP168822

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122591_RAW.tar 67.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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