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Series GSE122620 Query DataSets for GSE122620
Status Public on Nov 30, 2018
Title Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Spt6 is a histone chaperone that associates with RNA polymerase II and deposits nucleosomes in the wake of transcription. Although Spt6 has an essential function in nucleosome deposition, it is not known whether this function is regulated by post-translational modification. Here, we report that casein kinase II (CKII) phosphorylation of Spt6 directs nucleosome reassembly at the 5’ ends of a broad range of genes to prevent aberrant antisense transcription and enforce transcriptional directionality. Mechanistically, we show that interaction of Spt6 with Spn1 – a constitutive binding partner required for chromatin reassembly and full recruitment of Spt6 to genes is positively regulated by CKII phosphorylation of Spt6. Together, our study defines a previously unknown function for CKII phosphorylation in transcription, and further, highlights the importance of post-translational modification as a mechanism to fine-tune the functions of histone chaperones.
 
Overall design Understanding the role of Spt6 phosphorylation by Casein kinase 2 and the role of such phosphorylation in the regulation of biological functions of Spt6
 
Contributor(s) Dronamraju R, Kerschner JL, Peck SA, Hepperla AJ, Adams AT, Hughes KD, Aslam S, Davis IJ, Mosley AL, Strahl BD
Citation(s) 30566871
Submission date Nov 16, 2018
Last update date Mar 11, 2019
Contact name Jeremy M Simon
E-mail(s) jeremy_simon@med.unc.edu
Phone (919) 966-4050
Organization name University of North Carolina at Chapel Hill
Department Neuroscience
Street address 7018A Mary Ellen Jones Building
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (2)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
GPL21656 Illumina HiSeq 4000 (Saccharomyces cerevisiae)
Samples (31)
GSM3476453 spt6-1004 RNA Rep1
GSM3476454 spt6-1004 RNA Rep2
GSM3476455 spt6-1004 RNA Rep3
Relations
BioProject PRJNA505786
SRA SRP169073

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122620_RAW.tar 915.2 Mb (http)(custom) TAR (of BW)
GSE122620_WT_allRepsMerged_antisenseReads_R2.bw 2.9 Mb (ftp)(http) BW
GSE122620_WT_allRepsMerged_senseReads_R2.bw 31.6 Mb (ftp)(http) BW
GSE122620_spt6-1004.vs.WT.sense_diff_p0.05_DESeq2.txt.gz 35.1 Kb (ftp)(http) TXT
GSE122620_spt6-1004_allRepsMerged_antisenseReads_R2.bw 4.9 Mb (ftp)(http) BW
GSE122620_spt6-1004_allRepsMerged_senseReads_R2.bw 29.0 Mb (ftp)(http) BW
GSE122620_spt6-S8A.vs.WT.sense_diff_p0.05_DESeq2.txt.gz 1.4 Kb (ftp)(http) TXT
GSE122620_spt6-S8A_allRepsMerged_antisenseReads_R2.bw 2.8 Mb (ftp)(http) BW
GSE122620_spt6-S8A_allRepsMerged_senseReads_R2.bw 28.3 Mb (ftp)(http) BW
GSE122620_spt6-S8E.vs.WT.sense_diff_p0.05_DESeq2.txt.gz 201 b (ftp)(http) TXT
GSE122620_spt6-S8E_allRepsMerged_antisenseReads_R2.bw 2.7 Mb (ftp)(http) BW
GSE122620_spt6-S8E_allRepsMerged_senseReads_R2.bw 28.5 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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