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| Status |
Public on Mar 30, 2020 |
| Title |
A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (RNA-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Understanding the mechanisms that modulate T helper lymphocyte functions is crucial to decipher normal and pathogenic immune responses in humans. To identify molecular determinants influencing the pathogenicity of T cells, we separated ex vivo-isolated primary human memory T lymphocytes based on their ability to produce high levels of inflammatory cytokines. We found that the inflammatory, cytokine-producing phenotype of memory T lymphocytes was defined by a specific core gene signature and was mechanistically regulated by the constitutive activation of the NF-kB pathway and by the expression of the transcriptional repressor BHLHE40. BHLHE40 attenuated the expression of anti-inflammatory factors, including miR-146a, a negative regulator of NF-kB activation, and ZC3H12D, an RNase of the Regnase-1 family able to degrade inflammatory transcripts. Our data reveal a molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes, with the potential to contribute to disease.
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| Overall design |
Primary human T central memory cells (TEM) were obtained from 9 donors. TEM cells were PE-labelled for GM-CSF expression with a GM-CSF Cytokine Secretion Assay (Miltenyi Biotec) and sorted for PE+/-. Total RNA of 3 donors was combined and extracted.
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| Contributor(s) |
Emming S, Balestrieri C, Pollett S, Natoli G, Monticelli S |
| Citation(s) |
32205878 |
| |
| Submission date |
Nov 26, 2018 |
| Last update date |
Jun 29, 2020 |
| Contact name |
Chiara Balestrieri |
| E-mail(s) |
balestrieri.c@gmail.com
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| Organization name |
IRCCS San Raffaele Scientific Institute
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| Department |
Center for Omics Sciences
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| Street address |
Via Olgettina 58
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| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE122946 |
A molecular network regulating the proinflammatory phenotype of human memory T lymphocytes |
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| Relations |
| BioProject |
PRJNA506983 |
| SRA |
SRP170731 |
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