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Status |
Public on Mar 29, 2020 |
Title |
A molecular network regulating the proinflammatory phenotype of human memory T lymphocytes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Non-coding RNA profiling by array Expression profiling by array
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Summary |
Understanding the mechanisms that modulate T helper lymphocyte functions is crucial to decipher normal and pathogenic immune responses in humans. To identify molecular determinants influencing the pathogenicity of T cells, we separated ex vivo-isolated primary human memory T lymphocytes based on their ability to produce high levels of inflammatory cytokines. We found that the inflammatory, cytokine-producing phenotype of memory T lymphocytes was defined by a specific core gene signature and was mechanistically regulated by the constitutive activation of the NF-kB pathway and by the expression of the transcriptional repressor BHLHE40. BHLHE40 attenuated the expression of anti-inflammatory factors, including miR-146a, a negative regulator of NF-kB activation, and ZC3H12D, an RNase of the Regnase-1 family able to degrade inflammatory transcripts. Our data reveal a molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes, with the potential to contribute to disease.
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Overall design |
Refer to individual Series
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Citation(s) |
32205878 |
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Submission date |
Nov 26, 2018 |
Last update date |
Mar 31, 2020 |
Contact name |
Chiara Balestrieri |
E-mail(s) |
balestrieri.c@gmail.com
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Organization name |
IRCCS San Raffaele Scientific Institute
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Department |
Center for Omics Sciences
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Street address |
Via Olgettina 58
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City |
Milan |
ZIP/Postal code |
20132 |
Country |
Italy |
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Platforms (4)
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GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
GPL21847 |
nCounter Nanostring Human Immunology v2 (Catalog #115000061) |
GPL25243 |
NanoString nCounter miRNA Expression Panel |
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Samples (46)
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This SuperSeries is composed of the following SubSeries:
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GSE122940 |
A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (ChIP-Seq) |
GSE122941 |
A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (RNA-Seq) |
GSE122942 |
A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (Nanostring I) |
GSE122943 |
A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (Nanostring II) |
GSE122945 |
A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (Nanostring III) |
GSE134161 |
A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (ATAC-seq) |
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Relations |
BioProject |
PRJNA506974 |