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Series GSE122946 Query DataSets for GSE122946
Status Public on Mar 29, 2020
Title A molecular network regulating the proinflammatory phenotype of human memory T lymphocytes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Non-coding RNA profiling by array
Expression profiling by array
Summary Understanding the mechanisms that modulate T helper lymphocyte functions is crucial to decipher normal and pathogenic immune responses in humans. To identify molecular determinants influencing the pathogenicity of T cells, we separated ex vivo-isolated primary human memory T lymphocytes based on their ability to produce high levels of inflammatory cytokines. We found that the inflammatory, cytokine-producing phenotype of memory T lymphocytes was defined by a specific core gene signature and was mechanistically regulated by the constitutive activation of the NF-kB pathway and by the expression of the transcriptional repressor BHLHE40. BHLHE40 attenuated the expression of anti-inflammatory factors, including miR-146a, a negative regulator of NF-kB activation, and ZC3H12D, an RNase of the Regnase-1 family able to degrade inflammatory transcripts. Our data reveal a molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes, with the potential to contribute to disease.
 
Overall design Refer to individual Series
 
Citation(s) 32205878
Submission date Nov 26, 2018
Last update date Mar 31, 2020
Contact name Chiara Balestrieri
E-mail(s) balestrieri.c@gmail.com
Organization name IRCCS San Raffaele Scientific Institute
Department Center for Omics Sciences
Street address Via Olgettina 58
City Milan
ZIP/Postal code 20132
Country Italy
 
Platforms (4)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21847 nCounter Nanostring Human Immunology v2 (Catalog #115000061)
Samples (46)
GSM3488957 Jurkat.BHLHE40 Rep.1
GSM3488958 Jurkat.BHLHE40 Rep.2
GSM3488959 Jurkat.Empty Rep.1
This SuperSeries is composed of the following SubSeries:
GSE122940 A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (ChIP-Seq)
GSE122941 A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (RNA-Seq)
GSE122942 A molecular network regulating the pro-inflammatory phenotype of human memory T lymphocytes (Nanostring I)
Relations
BioProject PRJNA506974

Download family Format
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Supplementary file Size Download File type/resource
GSE122946_RAW.tar 1.5 Mb (http)(custom) TAR (of NARROWPEAK, TXT)
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