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Series GSE123017 Query DataSets for GSE123017
Status Public on Nov 28, 2018
Title Differential requirement for centriolar satellites in cilium formation and ciliary signaling among different vertebrate cells
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Centriolar satellites are an array of membrane-less granules that localize and move around the vertebrate centrosome/cilium complex. They have recently emerged as key regulators of the biogenesis and function of the centrosome/cilium-complex and their mutations are linked to ciliopathies. Although centriolar satellites are ubiquitous structures of the vertebrate cells, their precise function and molecular mechanism of action in different cell types remain poorly understood. Here, we generated kidney and retinal epithelial cells that lack centriolar satellites by genetically ablating their scaffolding protein PCM1 and investigated the cellular and molecular consequences of satellite loss in cells. We showed that centriolar satellites are required for cilium assembly, regulation of ciliary content, timely response to Hedgehog signals and three- dimensional epithelial cell organization, but not for cell proliferation, cell cycle progression and centriole duplication. Importantly, the requirement for centriolar satellites in cilium assembly varied between retinal and kidney epithelial cells and we identified the differences in the efficiency of targeting key ciliogenesis factors to the centrosome including Mib1 and Talpid3 as the likely molecular basis for this phenotypic variability. Quantitative global transcriptomic and proteomic profiling of satellite-less cells showed that loss of centriolar satellites does not lead to a major transcriptional response, but leads to a significant rearrangement of the global proteome. Together, our findings identify important roles for centriolar satellites in key cilium-related cellular processes through regulating the proteostasis and centrosomal/ciliary targeting of proteins and provide insight into the disease mechanisms of ciliopathies.
Overall design We performed global transcriptome analysis of wild type (WT) and PCM1 knock-out cells by using two different cells: human RPE1, and mouse IMCD3 cells. Deep sequencing technique was utilized via illumina HiSeq4000 platfrom in two biological replicates for each condition.
Contributor(s) Gul S, Kavakli IH
Citation(s) 31023719
Submission date Nov 27, 2018
Last update date Jun 20, 2019
Contact name seref gul
Organization name KOC University
Department Chemical and Biological Engineering
Street address Rumelifeneri Yolu, Sariyer
City Istanbul
ZIP/Postal code 34450
Country Turkey
Platforms (2)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM3490767 RPE1_WT_rep1
GSM3490768 RPE1_WT_rep2
GSM3490769 RPE1_PCM1_KO_rep1
BioProject PRJNA507257
SRA SRP170956

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Supplementary file Size Download File type/resource
GSE123017_IMCD3_gene_exp_diff.csv.gz 1.5 Mb (ftp)(http) CSV
GSE123017_IMCD3_gene_exp_diff_p0.5_f2.csv.gz 373 b (ftp)(http) CSV
GSE123017_RPE1_gene_exp_diff.csv.gz 1.7 Mb (ftp)(http) CSV
GSE123017_RPE1_gene_exp_diff_p0.5_f2.csv.gz 898 b (ftp)(http) CSV
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