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Series GSE123464 Query DataSets for GSE123464
Status Public on Feb 26, 2019
Title Clonal Analyses of Refractory Testicular Germ Cell Tumors
Organism Homo sapiens
Experiment type Genome variation profiling by array
Summary We used DNA content-based flow cytometry to distinguish and isolate nuclei of clonal tumor populations from primary and metastatic refractory testicular germ cell tumors (TGCTs) tissues. We then interrogated each sorted tumor populationwith whole genome aCGH and next generation sequencing (NGS). we have explored the clonal basis of refractory TGCT by investigating distinct tumor populations that were present in each tumor. Notably this included resected primary tissues and treatment refractory metastases that arose after high dose chemotherapy. These results provide new knowledge regarding the role of clonal selection and selected genomic lesions in the resistance to chemotherapy in TGCT within this exceptional cohort.
Overall design We applied a systematic approach combining DNA content flow cytometry, whole genome copy number and whole exome sequence analyses to interrogate tumor heterogeneity in primary and metastatic refractory testicular germ cell tumors (TGCTs). We identified both known and novel somatic copy number aberrations and mutations including candidate markers for platinum resistance that were present in a primary tumor of mixed histology and that remained after tandem autologous stem cell transplant..
Contributor(s) Lenkiewicz E, Malasi S, Stanton M, Slack J, Andrews P, Pagliaro L, Bryce AH, Barrett MT
Citation(s) 30870501
Submission date Dec 06, 2018
Last update date Apr 09, 2019
Contact name Michael Thomas Barrett
Phone 480-301-6736
Organization name Mayo Clinic Arizona
Department Molecular Pharmacology and Experimental Therapeutics
Street address 13400 East Shea Boulevard
City Scottsdale
State/province AZ
ZIP/Postal code 85259
Country USA
Platforms (1)
GPL19387 Agilent-021850 SurePrint G3 Human CGH Microarray (Probe Name version)
Samples (11)
GSM3504413 TGCT#1-3
GSM3504414 TGCT#1-5
GSM3504415 TGCT#1-A1
BioProject PRJNA508792

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123464_RAW.tar 473.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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