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Series GSE123515 Query DataSets for GSE123515
Status Public on Feb 28, 2019
Title Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 2]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The weekly turnover of the intestinal epithelium is driven by multipotent, Lgr5+, crypt base columnar cells (CBCs). In response to injury, however, Lgr5+ cells are lost but then re-emerge and are required for successful recovery. How these resurgent Lgr5+ stem cells arise is unclear. We transcriptionally profiled single cells from regenerating intestinal epithelia and identified a unique cell type we term the revival stem cell (rSC). rSCs are mutually exclusive to CBCs and are distinguished by elevated expression of cell survival and DNA repair genes. In homeostasis, rSCs are extremely rare, but nevertheless give rise to all the major cell types of the intestine including crypt-villus axes. After damage rSCs display a 20-fold, Yap-dependent, transient expansion, reconstitute the Lgr5+ CBC compartment and are required to regenerate a functional intestine. These studies define a unique stem cell phenotype that is mobilized by damage to reconstitute the intestinal epithelium.
 
Overall design Examination of regenerating mouse intestinal epithelium.
 
Contributor(s) Ayyaz A, Kumar S, Sangiorgi B, Ghoshal B, Gosio J, Ouladan S, Fink M, Barutcu S, Trcka D, Shen J, Chan K, Wrana JL, Gregorieff A
Citation(s) 31019301
Submission date Dec 07, 2018
Last update date May 26, 2019
Contact name Bibaswan Ghoshal
E-mail(s) bibaswan.ghoshal@gmail.com
Organization name Lunenfeld Tanenbaum Research Institute
Street address 600 University Avenue
City Toronto
ZIP/Postal code M5G 1X5
Country Canada
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (192)
GSM3505835 CluGFP+ [A01_Plate3]
GSM3505836 CluGFP+ [A02_Plate3]
GSM3505837 CluGFP+ [A03_Plate3]
This SubSeries is part of SuperSeries:
GSE123516 Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell
Relations
BioProject PRJNA508976
SRA SRP173202

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123515_processed_data.csv.gz 3.1 Mb (ftp)(http) CSV
GSE123515_raw_data.csv.gz 1.2 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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