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Series GSE124708 Query DataSets for GSE124708
Status Public on Jan 07, 2019
Title Hyperandrogenemia and western-style diet act synergistically on transcription and DNA methylation in visceral adipose tissue of a non-human primate model [RRBS]
Organism Macaca mulatta
Experiment type Methylation profiling by high throughput sequencing
Summary We have recently developed a nonhuman primate (NHP) model to address the effects of hyperandrogenemia (modestly elevated testosterone; T), a high-fat, obesogenic Western-style diet (WSD), or combined T+WSD. To address the molecular mechanisms underlying adipocyte dysfunction, visceral adipose tissue biopsies were analyzed for whole-genome gene expression and DNA methylation, using RNA-seq and reduced-representation bisulfite sequencing (RRBS), respectively. We show that the combination of T + WSD induces a significantly stronger transcriptional response than T or WSD alone, including regulation of genes whose expression is altered in adipose tissue of PCOS women. Gene expression patterns indicate alterations in cAMP, LXR/RXR, HIPPO, Wnt signaling and eicosanoid and retinol biosynthesis pathways. Analysis of DNA methylation shows that genes from the cAMP signaling pathway are also differentially methylated in T +WSD, which also globally resulted in a greater number of differentially methylated regions (DMRs) than T or WSD alone. Collectively, our results show that hyperandrogenemia and WSD exhibit synergistic effects on the visceral adipose tissue transcriptome and methylome, resulting in activation of biological processes that may contribute to the adipose tissue dysfunction characteristic of PCOS.
 
Overall design White Adipose Tissue biopsies from four experimental groups of rhesus macaques (control, testosterone, western style diet, and testosterone + western style diet) of 6 replicates per group, subjected to whole transcriptome analysis and reduced representation bisulfite sequencing.
 
Contributor(s) Carbone L, Davis BA, Varlamov O
Citation(s) 31848372
Submission date Jan 06, 2019
Last update date Dec 31, 2019
Contact name Brett Davis
E-mail(s) davibr@ohsu.edu
Organization name Oregon Health and Science University
Street address 3181 SW Sam Jackson Park Rd
City Portland
State/province Oregon
ZIP/Postal code 97239
Country USA
 
Platforms (1)
GPL21120 Illumina NextSeq 500 (Macaca mulatta)
Samples (24)
GSM3543717 C1 (RRBS)
GSM3543718 C2 (RRBS)
GSM3543719 C3 (RRBS)
This SubSeries is part of SuperSeries:
GSE124709 Hyperandrogenemia and western-style diet act synergistically on transcription and DNA methylation in visceral adipose tissue of a non-human primate model
Relations
BioProject PRJNA513191
SRA SRP176107

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Supplementary file Size Download File type/resource
GSE124708_ECP7.RRBS.processed.methRates.txt.gz 33.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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