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| Status |
Public on Jan 31, 2019 |
| Title |
Transcriptomic features of trophoblast lineage cells derived from BMP4-treated human induced pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We explored the trophoblast differentiation model using human induced pluripotent stem cells (hiPSCs) to gain new insights into the early development of human placenta. In our previous study, we have optimized culture conditions for hiPSC-derived trophoblasts. To identify the characteristics of trophoblast lineage cells derived from BMP4-treated hiPSCs, we collected cells using a pan-trophoblast marker, KRT7, with flow cytometry and performed comprehensive gene expression analysis.
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| Overall design |
For characterization of BMP4-treated hiPSCs, four types of hiPSCs were treated with 50ng/ml BMP4 for 10 days. Subsequently, KRT7-positive cells were purified from differentiated cells using flow cytometry and analyzed using DNA microarray.
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| Contributor(s) |
Tsuchida N, Kojima J, Fukuda A, Kawasaki T, Ito H, Kuji N, Isaka K, Nishi H, Umezawa A, Akutsu H |
| Citation(s) |
31675487 |
| |
| Submission date |
Jan 08, 2019 |
| Last update date |
Jun 09, 2021 |
| Contact name |
Nanae Tsuchida |
| E-mail(s) |
nt1030@tokyo-med.ac.jp
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| Phone |
81-3-3342-6111
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| Organization name |
Tokyo Medical University
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| Department |
Obstetrics and Gynecology
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| Street address |
6-7-1 Nishishinjuku
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| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-0023 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA513883 |
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