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Series GSE124901 Query DataSets for GSE124901
Status Public on Dec 31, 2019
Title Functional stem cell aging dictated by skin tissue microenvironment
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Organismal aging in mammals is manifested with architectural alteration and functional decline of multiple organs throughout the body. In aged skin, hairs are sparse, which has led to the hypothesis that the hair follicle stem cells (HFSCs) undergo epidermal differentiation during aging. Here, we employ single cell analysis to interrogate aging-related changes in the HFSCs. Unexpectedly, HFSCs maintain their lineage fidelity and show no signs of shifting to an epidermal fate. Despite maintaining lineage identity, HFSCs do show prevalent transcriptional changes in extracellular matrix genes. Of importance, these HFSC changes are accompanied by profound architectural perturbations in the aging stem cell niche. Upon surveying the dermis from young and aged skin, we also observe age-related changes in many non-epithelial cell types, including resident immune cells, sensory neurons, arrector pili muscles, and blood vessels – all of which have been previously associated with abilities to modulate hair follicle regeneration. Consistent with both intrinsic and extrinsic alterations in stem cell: niche communications, we find that in response to skin wounding, aged HFSCs repair the epidermis, but are defective in hair follicle regeneration. Intriguingly, whereas aged dermis cannot support young HFSCs, aged HFSCs can be rescued when supported by young dermis. Together, these findings favor a model where skin tissue microenvironment plays a dominant role in dictating the molecular properties and activities of HFSCs.
Overall design Young and aged murine hair follicle stem cells and those from interfollicular epidermis in the skin were purified and assayed for their genome-wide transcriptional (RNAseq) and open chromatin (ATACseq) landscape, and 10x genomics was used to survey single cell transcriptome in young and aged skin epithelium
Contributor(s) Ge Y, Fuchs E
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Submission date Jan 10, 2019
Last update date Jan 01, 2020
Contact name Yejing Ge
Organization name The Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (14)
GSM3558052 Sample 1_OdBu
GSM3558053 Sample 2_OdBu
GSM3558054 Sample 3_OdEpd
BioProject PRJNA514242
SRA SRP179070

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Supplementary file Size Download File type/resource
GSE124901_RAW.tar 276.2 Mb (http)(custom) TAR (of BED, MTX, TSV, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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