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Series GSE125427 Query DataSets for GSE125427
Status Public on Jan 22, 2020
Title Ascorbate oxidation primes rice plants for enhanced defence against root-knot nematode Meloidogyne graminicola through jasmonate/ethylene-activation
Organism Oryza sativa Japonica Group
Experiment type Expression profiling by high throughput sequencing
Summary Ascorbic acid (AA) is known to play a vital role in plant growth and detoxification of reactive oxygen species, however little is known about the significance of AA oxidation in plant defence against pathogens. • The role of ascorbate oxidation in rice defence against root-knot nematodes, Meloidogyne graminicola, was tested with application of AA, ascorbate oxidase (AO), dehydroascorbic acid (DHA), biosynthesis inhibitors and use of mutants. Transcriptome analysis was done on AO treated plants, and hormone measurements were executed to confirm the results. Biochemical analyses were used to study oxidative stress markers, including accumulation of H2O2, , malondialdehyde and AA/DHA.
Overall design The expression profile of ascorbate oxidase treated plants is compared to untreated plants both for plants infected with Meloidigyne graminicola as for uninfected plants. Three replicates for each group. Each sample was multiplexed in four subsamples.
Contributor(s) Richard S, Bruno V, Shahid S, Adelahu T, Raimund T, Michael F, Ashley H, Kristof D, Somnath P, Lieve G, Tina K
Citation(s) 32242224
Submission date Jan 22, 2019
Last update date Apr 22, 2020
Contact name Bruno Verstraeten
Organization name Ghent University
Street address /
City Ghent
State/province Oost-Vlaanderen
ZIP/Postal code 9000
Country Belgium
Platforms (1)
GPL26083 Illumina NextSeq 500 (Oryza sativa Japonica Group)
Samples (12)
GSM3573744 Control rep 1
GSM3573745 Control rep 2
GSM3573746 Control rep 3
BioProject PRJNA516355
SRA SRP181124

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125427_RAW.tar 4.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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