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Series GSE125585 Query DataSets for GSE125585
Status Public on Jan 25, 2019
Title MeCP2 regulates genome-wide gene expression through recognition of H3K27me3 [ChIP, MNase]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary MeCP2 plays a multifaceted role in gene expression regulation and chromatin organization. Interaction between MeCP2 and methylated DNA to regulate gene expression is well established. However, the widespread MeCP2 distribution suggests its additional interactions with chromatin. Here we show, by both biochemical and ChIP-seq analyses, that MeCP2 directly binds to nucleosome subunit proteins and is recruited to distinct chromatin regions where H3K27me3 is enriched. We further observed that the impact of MeCP2 on transcriptional changes is correlated with histone post-translational modification patterns. Our findings indicate that MeCP2 can be recruited to genomic loci via indirect binding and that interaction between MeCP2 and histone proteins plays a significant role in gene expression regulation.
Overall design Examination of two epigenetic markers from the olfactory epithelium in two biological samples. This dataset is created to supplement the MeCP2 ChIP-seq and MNase-seq datasets (GSE71126) generated by Rube HT. Nature Communications 2016.
Contributor(s) Lee W, Gong Q
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Submission date Jan 24, 2019
Last update date Mar 19, 2019
Contact name Wooje Lee
Organization name Chosun University
Department Cellular and Molecular Medicine
Street address 309, Pilmun-daero, Dong-gu
City Gwangju
ZIP/Postal code 61452
Country South Korea
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (5)
GSM3577862 GSM1827606 Input_WT_rep1
GSM3577863 GSM1827607 Input_WT_rep2
GSM3577864 GSM1827604 MeCP2_ChIP_WT_rep1
This SubSeries is part of SuperSeries:
GSE122366 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3
BioProject PRJNA516876
SRA SRP181872

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Supplementary file Size Download File type/resource 699.5 Mb (ftp)(http) BW
GSE125585_MNase_Peaks_by_PING.bed.gz 66.1 Mb (ftp)(http) BED 636.7 Mb (ftp)(http) BW 649.6 Mb (ftp)(http) BW
GSE125585_MeCP2_Peaks_by_PING.bed.gz 36.2 Mb (ftp)(http) BED
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