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Series GSE125629 Query DataSets for GSE125629
Status Public on Jan 25, 2019
Title A HOTAIR regulatory element modulates glioma cell sensitivity to temozolomide through long-range regulation of multiple target genes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Temozolomide (TMZ) is a frequently used chemotherapy for glioma; however, chemoresistance is a major problem limiting its effectiveness. Thus knowledge of mechanisms underlying this outcome could improve patient prognosis. Here, we report that deletion of a regulatory element in the HOTAIR locus increases glioma cell sensitivity to TMZ and alters transcription of multiple genes. Analysis of a combination of RNA-seq, Capture HiC and patient survival data suggests that CALCOCO1 and ZC3H10 are target genes repressed by the HOTAIR regulatory element and that both function in regulating glioma cell sensitivity to TMZ. Rescue experiments and TAD analysis based on HiC data confirmed this hypothesis. We propose a new regulatory mechanism governing glioma cell TMZ sensitivity.
Overall design BioProject: PRJNA516515. SRA Study accession:SRP181265 .
Contributor(s) Zhang L, Lu W
Citation(s) 31953347
BioProject PRJNA516515
Submission date Jan 24, 2019
Last update date Aug 10, 2021
Contact name Bohan Chen
Organization name Nankai University
Street address NO.94 Weijin Road
City Tianjin
ZIP/Postal code 300071
Country China
Platforms (2)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (10)
GSM3579137 DMSO_HOTAIR-KO1 replicate 1
GSM3579138 DMSO_HOTAIR-KO1 replicate 2
GSM3579139 TMZ_HOTAIR-KO1 replicate 1
SRA SRP181265

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125629_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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