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Series GSE125727 Query DataSets for GSE125727
Status Public on Apr 30, 2019
Title Microarray data for Bhlhe40-sufficient and -deficient resident macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Most tissue-resident macrophage populations develop during embryogenesis, self-renew in the steady state, and can expand during type 2 immunity. Whether shared mechanisms regulate macrophage proliferation in homeostasis and disease is unclear. We found that the transcription factor Bhlhe40 was cell-intrinsically required in large peritoneal macrophages (LPMs) for self-renewal and maintenance, but was not required in other resident macrophages. Bhlhe40 was selectively necessary in LPMs for proliferation, but not polarization, in response to IL-4. During a helminth infection,
Bhlhe40 was required for normal LPM cell cycling. Bhlhe40 repressed the expression of Maf and Mafb and directly promoted expression of cell cycle-related transcripts to enable proliferation of LPMs. Genomic sites bound by Bhlhe40 in LPMs included some co-bound by the macrophage lineage-determining factor PU.1 and others uniquely bound by Bhlhe40, including Maf and cell cycle-related loci. Our findings demonstrate a tissue-specific control mechanism regulating resident macrophage proliferation in homeostasis and type 2 immunity.
Overall design Bhlhe40+/+=Bhlhe40WT, Bhlhe40-/-=Bhlhe40KO, LysM-Cre+ Bhlhe40fl/fl=Bhlhe40LysM. AMs, 2 Bhlhe40+/+ and 2 Bhlhe40-/- samples sorted from naïve mice. Naïve LPMs, 3 Bhlhe40+/+ and 3 Bhlhe40-/- samples sorted from naïve mice. IL-4c-stimulated LPMs, 2 Bhlhe40+/+, 2 Bhlhe40-/-, and 2 LysM-Cre+ Bhlhe40fl/fl samples sorted from IL-4c-treated mice
Contributor(s) Jarjour NN, Edelson BT
Citation(s) 31061528
Submission date Jan 28, 2019
Last update date Jul 30, 2019
Contact name Brian T Edelson
Organization name Washington University in St. Louis
Department Pathology and Immunology
Lab Rm. 8304
Street address 425 S. Euclid Ave.
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (16)
GSM3581087 naïve Bhlhe40WT AM rep1
GSM3581088 naïve Bhlhe40WT AM rep2
GSM3581089 naïve Bhlhe40KO AM rep1
This SubSeries is part of SuperSeries:
GSE125730 Bhlhe40 mediates tissue-specific control of macrophage proliferation in homeostasis and type 2 immunity
BioProject PRJNA517406

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125727_RAW.tar 58.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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