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Series GSE125785 Query DataSets for GSE125785
Status Public on Jan 26, 2022
Title Transcriptomics analysis of gene expression in wildtype and FTO knockout mouse liver
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Fto conditional knockout mutation was generated using phage-based Escherichia coli homologous recombination systems. Construct targeting the third exon of mouse Fto gene was introduced into ES cell by homologous recombination. The mice with homozygous targeted allele were crossed to Albumin-Cre for deletion the third exon of mouse Fto gene in liver. Total RNA was isolated from Liver Tissue using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq3000 (Illumina) in paired-read mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used.
Overall design Examination of gene expressive levels in wildtype and FTO knockout mouse liver
Contributor(s) Peng S, Xiao W, Sun B, Huang N, Yang Y
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Submission date Jan 28, 2019
Last update date Jan 28, 2022
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM3581866 Fto WT RNA-seq rep1
GSM3581867 Fto WT RNA-seq rep2
GSM3581868 Fto Knockout RNA-seq rep1
BioProject PRJNA517498
SRA SRP182719

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125785_RAW.tar 570.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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